Commercial Bovine Proteoglycan Is Highly Arthritogenic and Can Be Used as an Alternative Antigen Source for PGIA Model
Joint Authors
Balbino, Bianca
Chiuso-Minicucci, Fernanda
Ishikawa, Larissa Lumi Watanabe
Colavite, Priscila Maria
Zorzella-Pezavento, Sofia Fernanda Gonçalves
França, Thais Graziela Donegá
Sartori, Alexandrina
da Rosa, Larissa Camargo
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-05-27
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Rheumatoid arthritis (RA) is the most common systemic autoimmune disease.
It affects mainly the joints, causing synovitis, cartilage destruction, and bone erosion.
Many experimental models are used to study the mechanisms involved in immunopathogenesis and new therapies for this disease.
Proteoglycan-induced arthritis (PGIA) is a widely used model based on the cross-reactivity of injected foreign (usually human) PG and mice self-PG.
Considering the complexity of the extraction and purification of human PG, in this study we evaluated the arthritogenicity of bovine PG that is commercially available.
Bovine PG was highly arthritogenic, triggering 100% incidence of arthritis in female BALB/c retired breeder mice.
Animals immunized with bovine PG presented clinical symptoms and histopathological features similar to human RA and other experimental models.
Moreover, bovine PG immunization determined higher levels of proinflammatory and anti-inflammatory cytokines in arthritic mice compared to healthy ones.
As expected, only the arthritic group produced IgG1 and IgG2a antibodies against PG.
Thus, commercial bovine PG can be used as an alternative antigenic source to PGIA for the study of many RA aspects, including the immunopathogenesis of the disease and also the development of new therapies.
American Psychological Association (APA)
Ishikawa, Larissa Lumi Watanabe& Colavite, Priscila Maria& da Rosa, Larissa Camargo& Balbino, Bianca& França, Thais Graziela Donegá& Zorzella-Pezavento, Sofia Fernanda Gonçalves…[et al.]. 2014. Commercial Bovine Proteoglycan Is Highly Arthritogenic and Can Be Used as an Alternative Antigen Source for PGIA Model. BioMed Research International،Vol. 2014, no. 2014, pp.1-12.
https://search.emarefa.net/detail/BIM-449632
Modern Language Association (MLA)
Ishikawa, Larissa Lumi Watanabe…[et al.]. Commercial Bovine Proteoglycan Is Highly Arthritogenic and Can Be Used as an Alternative Antigen Source for PGIA Model. BioMed Research International No. 2014 (2014), pp.1-12.
https://search.emarefa.net/detail/BIM-449632
American Medical Association (AMA)
Ishikawa, Larissa Lumi Watanabe& Colavite, Priscila Maria& da Rosa, Larissa Camargo& Balbino, Bianca& França, Thais Graziela Donegá& Zorzella-Pezavento, Sofia Fernanda Gonçalves…[et al.]. Commercial Bovine Proteoglycan Is Highly Arthritogenic and Can Be Used as an Alternative Antigen Source for PGIA Model. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-12.
https://search.emarefa.net/detail/BIM-449632
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-449632