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Process Improvement by Eliminating Mixing of Whole Blood Units after an Overnight Hold Prior to Component Production Using the Buffy Coat Method
Joint Authors
Levin, Elena
Yi, Qi-Long
Sheffield, William P.
Stewart, Tamiko
Jenkins, Craig
Hansen, Adele
Mastronardi, Cherie
Schubert, Peter
Lefresne, Wanda
Acker, Jason P.
Bhakta, Varsha
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-06-05
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
The elimination of a thorough manual mixing of whole blood (WB) which takes place following the overnight hold, but before the first centrifugation step, during buffy coat component production at Canadian Blood Services (CBS) was investigated.
WB was pooled after donation and split.
Pairs of platelet, red blood cell (RBC), and plasma components were produced, with half using the standard method and half using a method in which the mixing step was eliminated.
Quality assessments included yield, pH, CD62P expression and morphology for platelets, hemoglobin, hematocrit, hemolysis, and supernatant K+ for RBCs, and volume and factor VIII activity levels for plasma.
All components, produced using either method, met CBS quality control criteria.
There were no significant differences in platelet yield between components produced with and without mixing.
A significant difference was seen for RBC hemolysis at expiry (P=0.03), but for both groups, levels met quality control requirements.
Noninferiority of components produced without mixing was confirmed for all parameters.
Manual mixing is laborious and has a risk of repetitive strain for production staff and its significance is unclear.
Elimination of this step will improve process efficiencies without compromising quality.
American Psychological Association (APA)
Mastronardi, Cherie& Schubert, Peter& Levin, Elena& Bhakta, Varsha& Yi, Qi-Long& Hansen, Adele…[et al.]. 2013. Process Improvement by Eliminating Mixing of Whole Blood Units after an Overnight Hold Prior to Component Production Using the Buffy Coat Method. Journal of Blood Transfusion،Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-450197
Modern Language Association (MLA)
Mastronardi, Cherie…[et al.]. Process Improvement by Eliminating Mixing of Whole Blood Units after an Overnight Hold Prior to Component Production Using the Buffy Coat Method. Journal of Blood Transfusion No. 2013 (2013), pp.1-7.
https://search.emarefa.net/detail/BIM-450197
American Medical Association (AMA)
Mastronardi, Cherie& Schubert, Peter& Levin, Elena& Bhakta, Varsha& Yi, Qi-Long& Hansen, Adele…[et al.]. Process Improvement by Eliminating Mixing of Whole Blood Units after an Overnight Hold Prior to Component Production Using the Buffy Coat Method. Journal of Blood Transfusion. 2013. Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-450197
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-450197