Production and Biochemical Characterization of a High Maltotetraose (G4) Producing Amylase from Pseudomonas stutzeri AS22
Joint Authors
Maalej, Hana
Ben Ayed, Hanen
Nasri, Moncef
Ghorbel-Bellaaj, Olfa
Hmidet, Noomen
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-05-25
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Amylase production and biochemical characterization of the crude enzyme preparation from Pseudomonas stutzeri AS22 were evaluated.
The highest α-amylase production was achieved after 24 hours of incubation in a culture medium containing 10 g/L potato starch and 5 g/L yeast extract, with initial pH 8.0 at 30°C under continuous agitation at 200 rpm.
The optimum temperature and pH for the crude α-amylase activity were 60°C and 8.0, respectively.
The effect of different salts was evaluated and it was found that both α-amylase production and activity were Ca2+-dependent.
The amylolytic preparation was found to catalyze exceptionally the formation of very high levels of maltotetraose from starch (98%, w/w) in the complete absence of glucose since the initial stages of starch hydrolysis (15 min) and hence would have a potential application in the manufacturing of maltotetraose syrups.
American Psychological Association (APA)
Maalej, Hana& Ben Ayed, Hanen& Ghorbel-Bellaaj, Olfa& Nasri, Moncef& Hmidet, Noomen. 2014. Production and Biochemical Characterization of a High Maltotetraose (G4) Producing Amylase from Pseudomonas stutzeri AS22. BioMed Research International،Vol. 2014, no. 2014, pp.1-11.
https://search.emarefa.net/detail/BIM-450276
Modern Language Association (MLA)
Maalej, Hana…[et al.]. Production and Biochemical Characterization of a High Maltotetraose (G4) Producing Amylase from Pseudomonas stutzeri AS22. BioMed Research International No. 2014 (2014), pp.1-11.
https://search.emarefa.net/detail/BIM-450276
American Medical Association (AMA)
Maalej, Hana& Ben Ayed, Hanen& Ghorbel-Bellaaj, Olfa& Nasri, Moncef& Hmidet, Noomen. Production and Biochemical Characterization of a High Maltotetraose (G4) Producing Amylase from Pseudomonas stutzeri AS22. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-11.
https://search.emarefa.net/detail/BIM-450276
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-450276