Osthole Attenuates Doxorubicin-Induced Apoptosis in PC12 Cells through Inhibition of Mitochondrial Dysfunction and ROS Production
Joint Authors
Mohammadi-Motlagh, Hamid-Reza
Hosseinzadeh, Leila
Shokoohinia, Yalda
Mostafaie, Ali
Moieni-Arya, Maryam
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-06-12
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Doxorubicin (DOX) is a potent, broad-spectrum chemotherapeutic drug used for treatment of several types of cancers.
Despite its effectiveness, it has a wide range of toxic side effects, many of which most likely result from its inherent prooxidant activity.
It has been reported that DOX has toxic effects on normal tissues, including brain tissue.
In the current study, we investigated the protective effect of osthole isolated from Prangos ferulacea (L.) Lindl.
on oxidative stress and apoptosis induced by DOX in PC12 as a neuronal model cell line.
PC12 cells were pretreated with osthole 2 h after treatment with different concentrations of DOX.
24 h later, the cell viability, mitochondrial membrane potential (MMP), the activity of caspase-3, the expression ratio of Bax/Bcl-2, and the generation of intracellular ROS were detected.
We found that pretreatment with osthole on PC12 cells significantly reduced the loss of cell viability, the activity of caspase-3, the increase in Bax/Bcl-2 ratio, and the generation of intracellular ROS induced by DOX.
Moreover, pretreatment with osthole led to an increase in MMP in PC12 cells.
In conclusion, our results indicated that pretreatment with nontoxic concentrations of osthole protected PC12 cells from DOX-mediated apoptosis by inhibition of ROS production.
American Psychological Association (APA)
Shokoohinia, Yalda& Hosseinzadeh, Leila& Moieni-Arya, Maryam& Mostafaie, Ali& Mohammadi-Motlagh, Hamid-Reza. 2014. Osthole Attenuates Doxorubicin-Induced Apoptosis in PC12 Cells through Inhibition of Mitochondrial Dysfunction and ROS Production. BioMed Research International،Vol. 2014, no. 2014, pp.1-7.
https://search.emarefa.net/detail/BIM-450312
Modern Language Association (MLA)
Shokoohinia, Yalda…[et al.]. Osthole Attenuates Doxorubicin-Induced Apoptosis in PC12 Cells through Inhibition of Mitochondrial Dysfunction and ROS Production. BioMed Research International No. 2014 (2014), pp.1-7.
https://search.emarefa.net/detail/BIM-450312
American Medical Association (AMA)
Shokoohinia, Yalda& Hosseinzadeh, Leila& Moieni-Arya, Maryam& Mostafaie, Ali& Mohammadi-Motlagh, Hamid-Reza. Osthole Attenuates Doxorubicin-Induced Apoptosis in PC12 Cells through Inhibition of Mitochondrial Dysfunction and ROS Production. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-7.
https://search.emarefa.net/detail/BIM-450312
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-450312