Production, Purification, and Characterization of Polygalacturonase from Mucor circinelloides ITCC 6025
Joint Authors
Singh, Smarika
Pahwa, Roma
Thakur, Akhilesh
Gupta, Reena
Source
Issue
Vol. 2010, Issue 2010 (31 Dec. 2010), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-06-01
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Mucor circinelloides produced an extracellular polygalacturonase enzyme, the production of which was enhanced when various production parameters were optimized.
Maximum polygalacturonase (PGase) activity was obtained in 48 h at 30∘C and pH 4.0 with pectin methyl ester (1% w/v) as carbon source and a combination of casein hydrolysate (0.1% w/v) and yeast extract (0.1% w/v) as nitrogen source.
The enzyme was purified to homogeneity (13.3-fold) by Sephacryl S-100 gel-filtration chromatography.
Its molecular weight was 66 kDa on SDS-PAGE.
The enzyme was found to have Km and Vmax values of 2.2 mM and 4.81 IU/ml at 0.1% to 0.5% (w/v) concentration of the substrate.
The addition of phenolic acids (0.05 mM), metal ions such as Mn+2, Co+2, Mg+2, Fe+3, Al+3, Hg+2, and Cu+2, and thiols had inhibitory effect on the enzyme.
The enzyme showed maximum activity in the presence of polygalacturonic acid (0.1% w/v) at pH 5.5 and 42∘C.
American Psychological Association (APA)
Thakur, Akhilesh& Pahwa, Roma& Singh, Smarika& Gupta, Reena. 2010. Production, Purification, and Characterization of Polygalacturonase from Mucor circinelloides ITCC 6025. Enzyme Research،Vol. 2010, no. 2010, pp.1-7.
https://search.emarefa.net/detail/BIM-451499
Modern Language Association (MLA)
Thakur, Akhilesh…[et al.]. Production, Purification, and Characterization of Polygalacturonase from Mucor circinelloides ITCC 6025. Enzyme Research No. 2010 (2010), pp.1-7.
https://search.emarefa.net/detail/BIM-451499
American Medical Association (AMA)
Thakur, Akhilesh& Pahwa, Roma& Singh, Smarika& Gupta, Reena. Production, Purification, and Characterization of Polygalacturonase from Mucor circinelloides ITCC 6025. Enzyme Research. 2010. Vol. 2010, no. 2010, pp.1-7.
https://search.emarefa.net/detail/BIM-451499
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-451499