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Gene Profiling of Aortic Valve Interstitial Cells under Elevated Pressure Conditions : Modulation of Inflammatory Gene Networks
Joint Authors
Muir, William M.
Pregonero Gamez, Carol A.
Warnock, James N.
Tang, Juliet
Koback, Daniel
Burgess, Shane C.
Nanduri, Bindu
Source
International Journal of Inflammation
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-08-18
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
The study aimed to identify mechanosensitive pathways and gene networks that are stimulated by elevated cyclic pressure in aortic valve interstitial cells (VICs) and lead to detrimental tissue remodeling and/or pathogenesis.
Porcine aortic valve leaflets were exposed to cyclic pressures of 80 or 120 mmHg, corresponding to diastolic transvalvular pressure in normal and hypertensive conditions, respectively.
Linear, two-cycle amplification of total RNA, followed by microarray was performed for transcriptome analysis (with qRT-PCR validation).
A combination of systems biology modeling and pathway analysis identified novel genes and molecular mechanisms underlying the biological response of VICs to elevated pressure.
56 gene transcripts related to inflammatory response mechanisms were differentially expressed.
TNF-α, IL-1α, and IL-1β were key cytokines identified from the gene network model.
Also of interest was the discovery that pentraxin 3 (PTX3) was significantly upregulated under elevated pressure conditions (41-fold change).
In conclusion, a gene network model showing differentially expressed inflammatory genes and their interactions in VICs exposed to elevated pressure has been developed.
This system overview has detected key molecules that could be targeted for pharmacotherapy of aortic stenosis in hypertensive patients.
American Psychological Association (APA)
Warnock, James N.& Nanduri, Bindu& Pregonero Gamez, Carol A.& Tang, Juliet& Koback, Daniel& Muir, William M.…[et al.]. 2011. Gene Profiling of Aortic Valve Interstitial Cells under Elevated Pressure Conditions : Modulation of Inflammatory Gene Networks. International Journal of Inflammation،Vol. 2011, no. 2011, pp.1-10.
https://search.emarefa.net/detail/BIM-452038
Modern Language Association (MLA)
Warnock, James N.…[et al.]. Gene Profiling of Aortic Valve Interstitial Cells under Elevated Pressure Conditions : Modulation of Inflammatory Gene Networks. International Journal of Inflammation No. 2011 (2011), pp.1-10.
https://search.emarefa.net/detail/BIM-452038
American Medical Association (AMA)
Warnock, James N.& Nanduri, Bindu& Pregonero Gamez, Carol A.& Tang, Juliet& Koback, Daniel& Muir, William M.…[et al.]. Gene Profiling of Aortic Valve Interstitial Cells under Elevated Pressure Conditions : Modulation of Inflammatory Gene Networks. International Journal of Inflammation. 2011. Vol. 2011, no. 2011, pp.1-10.
https://search.emarefa.net/detail/BIM-452038
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-452038