Effects of Chlorophyll-Derived Efflux Pump Inhibitor Pheophorbide a and Pyropheophorbide a on Growth and Macrolide Antibiotic Resistance of Indicator and Anaerobic Swine Manure Bacteria

Joint Authors

Whitehead, Terence R.
Cotta, Michael A.
Rasmussen, Mark A.
Kraatz, Mareike
Berhow, Mark A.

Source

International Journal of Antibiotics

Issue

Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-14, 14 p.

Publisher

Hindawi Publishing Corporation

Publication Date

2014-02-11

Country of Publication

Egypt

No. of Pages

14

Main Subjects

Pharmacology

Abstract EN

Natural plant compounds, such as the chlorophyll a catabolites pheophorbide a (php) and pyropheophorbide a (pyp), are potentially active in the gastrointestinal tracts and manure of livestock as antimicrobial resistance-modifying agents through inhibition of bacterial efflux pumps.

To investigate whether php, a known efflux pump inhibitor, and pyp influence bacterial resistance, we determined their long-term effects on the MICs of erythromycin for reference strains of clinically relevant indicator bacteria with macrolide or multidrug resistance efflux pumps.

Pyp reduced the final MIC endpoint for Staphylococcus (S.) aureus and Escherichia (E.) coli by up to 1536 and 1024 μg erythromycin mL−1 or 1.4- and 1.2-fold, respectively.

Estimation of growth parameters of S.

aureus revealed that pyp exerted an intrinsic inhibitory effect under anaerobic conditions and was synergistically active, thereby potentiating the effect of erythromycin and partially reversing high-level erythromycin resistance.

Anaerobe colony counts of total and erythromycin-resistant bacteria from stored swine manure samples tended to be lower in the presence of pyp.

Tylosin, php, and pyp were not detectable by HPLC in the manure or medium.

This is the first study showing that pyp affects growth and the level of sensitivity to erythromycin of S.

aureus, E.

coli, and anaerobic manure bacteria.

American Psychological Association (APA)

Kraatz, Mareike& Whitehead, Terence R.& Cotta, Michael A.& Berhow, Mark A.& Rasmussen, Mark A.. 2014. Effects of Chlorophyll-Derived Efflux Pump Inhibitor Pheophorbide a and Pyropheophorbide a on Growth and Macrolide Antibiotic Resistance of Indicator and Anaerobic Swine Manure Bacteria. International Journal of Antibiotics،Vol. 2014, no. 2014, pp.1-14.
https://search.emarefa.net/detail/BIM-452740

Modern Language Association (MLA)

Kraatz, Mareike…[et al.]. Effects of Chlorophyll-Derived Efflux Pump Inhibitor Pheophorbide a and Pyropheophorbide a on Growth and Macrolide Antibiotic Resistance of Indicator and Anaerobic Swine Manure Bacteria. International Journal of Antibiotics No. 2014 (2014), pp.1-14.
https://search.emarefa.net/detail/BIM-452740

American Medical Association (AMA)

Kraatz, Mareike& Whitehead, Terence R.& Cotta, Michael A.& Berhow, Mark A.& Rasmussen, Mark A.. Effects of Chlorophyll-Derived Efflux Pump Inhibitor Pheophorbide a and Pyropheophorbide a on Growth and Macrolide Antibiotic Resistance of Indicator and Anaerobic Swine Manure Bacteria. International Journal of Antibiotics. 2014. Vol. 2014, no. 2014, pp.1-14.
https://search.emarefa.net/detail/BIM-452740

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references

Record ID

BIM-452740