Differential Gene Expression Segregates Cattle Confirmed Positive for Bovine Tuberculosis from Antemortem Tuberculosis Test-False Positive Cattle Originating from Herds Free of Bovine Tuberculosis
Joint Authors
Steibel, Juan P.
Coussens, Paul M.
Grooms, Daniel L.
Bolin, Steven R.
Lim, Ailam
Source
Veterinary Medicine International
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-06-04
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Antemortem tests for bovine tuberculosis (bTB) currently used in the US measure cell-mediated immune responses against Mycobacterium bovis.
Postmortem tests for bTB rely on observation of gross and histologic lesions of bTB, followed by bacterial isolation or molecular diagnostics.
Cumulative data from the state of Michigan indicates that 98 to 99% of cattle that react positively in antemortem tests are not confirmed positive for bTB at postmortem examination.
Understanding the fundamental differences in gene regulation between antemortem test-false positive cattle and cattle that have bTB may allow identification of molecular markers that can be exploited to better separate infected from noninfected cattle.
An immunospecific cDNA microarray was used to identify altered gene expression (P≤0.01) of 122 gene features between antemortem test-false positive cattle and bTB-infected cattle following a 4-hour stimulation of whole blood with tuberculin.
Further analysis using quantitative real-time PCR assays validated altered expression of 8 genes that had differential power (adj P≤0.05) to segregate cattle confirmed positive for bovine tuberculosis from antemortem tuberculosis test-false positive cattle originating from herds free of bovine tuberculosis.
American Psychological Association (APA)
Lim, Ailam& Steibel, Juan P.& Coussens, Paul M.& Grooms, Daniel L.& Bolin, Steven R.. 2012. Differential Gene Expression Segregates Cattle Confirmed Positive for Bovine Tuberculosis from Antemortem Tuberculosis Test-False Positive Cattle Originating from Herds Free of Bovine Tuberculosis. Veterinary Medicine International،Vol. 2012, no. 2012, pp.1-12.
https://search.emarefa.net/detail/BIM-453370
Modern Language Association (MLA)
Lim, Ailam…[et al.]. Differential Gene Expression Segregates Cattle Confirmed Positive for Bovine Tuberculosis from Antemortem Tuberculosis Test-False Positive Cattle Originating from Herds Free of Bovine Tuberculosis. Veterinary Medicine International No. 2012 (2012), pp.1-12.
https://search.emarefa.net/detail/BIM-453370
American Medical Association (AMA)
Lim, Ailam& Steibel, Juan P.& Coussens, Paul M.& Grooms, Daniel L.& Bolin, Steven R.. Differential Gene Expression Segregates Cattle Confirmed Positive for Bovine Tuberculosis from Antemortem Tuberculosis Test-False Positive Cattle Originating from Herds Free of Bovine Tuberculosis. Veterinary Medicine International. 2012. Vol. 2012, no. 2012, pp.1-12.
https://search.emarefa.net/detail/BIM-453370
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-453370