Identification of Differentially Expressed Gene after Femoral Fracture via Microarray Profiling
Author
Source
International Journal of Genomics
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-07-08
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Biology
Abstract EN
We aimed to investigate differentially expressed genes (DEGs) in different stages after femoral fracture based on rat models, providing the basis for the treatment of sport-related fractures.
Gene expression data GSE3298 was downloaded from Gene Expression Omnibus (GEO), including 16 chips.
All femoral fracture samples were classified into earlier fracture stage and later fracture stage.
Total 87 DEGs simultaneously occurred in two stages, of which 4 genes showed opposite expression tendency.
Out of the 4 genes, Rest and Cst8 were hub nodes in protein-protein interaction (PPI) network.
The GO (Gene Ontology) function enrichment analysis verified that nutrition supply related genes were enriched in the earlier stage and neuron growth related genes were enriched in the later stage.
Calcium signaling pathway was the most significant pathway in earlier stage; in later stage, DEGs were enriched into 2 neurodevelopment-related pathways.
Analysis of Pearson's correlation coefficient showed that a total of 3,300 genes were significantly associated with fracture time, none of which was overlapped with identified DEGs.
This study suggested that Rest and Cst8 might act as potential indicators for fracture healing.
Calcium signaling pathway and neurodevelopment-related pathways might be deeply involved in bone healing after femoral fracture.
American Psychological Association (APA)
Zhong, Donggen. 2014. Identification of Differentially Expressed Gene after Femoral Fracture via Microarray Profiling. International Journal of Genomics،Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-454681
Modern Language Association (MLA)
Zhong, Donggen. Identification of Differentially Expressed Gene after Femoral Fracture via Microarray Profiling. International Journal of Genomics No. 2014 (2014), pp.1-9.
https://search.emarefa.net/detail/BIM-454681
American Medical Association (AMA)
Zhong, Donggen. Identification of Differentially Expressed Gene after Femoral Fracture via Microarray Profiling. International Journal of Genomics. 2014. Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-454681
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-454681