AP-1IRF-3 Targeted Anti-Inflammatory Activity of Andrographolide Isolated from Andrographis paniculata
Joint Authors
Shen, Ting
Yang, Woo Seok
Yi, Young-Su
Kim, Mi-Yeon
Kim, Jong Heon
Kim, Kyung-Woon
Poo, Haryoung
Cho, Jae Youl
Rhee, Man Hee
Sung, Gi-Ho
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-16, 16 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-06-06
Country of Publication
Egypt
No. of Pages
16
Main Subjects
Abstract EN
Andrographolide (AG) is an abundant component of plants of the genus Andrographis and has a number of beneficial properties including neuroprotective, anticancer, anti-inflammatory, and antidiabetic effects.
Despite numerous pharmacological studies, the precise mechanism of AG is still ambiguous.
Thus, in the present study, we investigated the molecular mechanisms of AG and its target proteins as they pertain to anti-inflammatory responses.
AG suppressed the production of nitric oxide (NO) and prostaglandin E2 (PGE2), as well as the mRNA abundance of inducible NO synthase (iNOS), tumor necrosis factor-alpha (TNF-α), cyclooxygenase (COX)-2, and interferon-beta (IFN-β) in a dose-dependent manner in both lipopolysaccharide- (LPS-) activated RAW264.7 cells and peritoneal macrophages.
AG also substantially ameliorated the symptoms of LPS-induced hepatitis and EtOH/HCl-induced gastritis in mice.
Based on the results of luciferase reporter gene assays, kinase assays, and measurement of nuclear levels of transcription factors, the anti-inflammatory effects of AG were found to be clearly mediated by inhibition of both (1) extracellular signal-regulated kinase (ERK)/activator protein (AP)-1 and (2) IκB kinase ε (IKKε)/interferon regulatory factor (IRF)-3 pathways.
In conclusion, we detected a novel molecular signaling pathway by which AG can suppress inflammatory responses.
Thus, AG is a promising anti-inflammatory drug with two pharmacological targets.
American Psychological Association (APA)
Shen, Ting& Yang, Woo Seok& Yi, Young-Su& Sung, Gi-Ho& Rhee, Man Hee& Poo, Haryoung…[et al.]. 2013. AP-1IRF-3 Targeted Anti-Inflammatory Activity of Andrographolide Isolated from Andrographis paniculata. Evidence-Based Complementary and Alternative Medicine،Vol. 2013, no. 2013, pp.1-16.
https://search.emarefa.net/detail/BIM-454866
Modern Language Association (MLA)
Shen, Ting…[et al.]. AP-1IRF-3 Targeted Anti-Inflammatory Activity of Andrographolide Isolated from Andrographis paniculata. Evidence-Based Complementary and Alternative Medicine No. 2013 (2013), pp.1-16.
https://search.emarefa.net/detail/BIM-454866
American Medical Association (AMA)
Shen, Ting& Yang, Woo Seok& Yi, Young-Su& Sung, Gi-Ho& Rhee, Man Hee& Poo, Haryoung…[et al.]. AP-1IRF-3 Targeted Anti-Inflammatory Activity of Andrographolide Isolated from Andrographis paniculata. Evidence-Based Complementary and Alternative Medicine. 2013. Vol. 2013, no. 2013, pp.1-16.
https://search.emarefa.net/detail/BIM-454866
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-454866