Enhanced Neural Cell Adhesion and Neurite Outgrowth on Graphene-Based Biomimetic Substrates
Joint Authors
Hong, Suck Won
Lee, Jong Ho
Han, Dong-Wook
Lee, Mi Hee
Hwang, Yu-Shik
Hwang, Eun Young
Kang, Seok Hee
Park, Jong-Chul
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-01-30
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Neural cell adhesion and neurite outgrowth were examined on graphene-based biomimetic substrates.
The biocompatibility of carbon nanomaterials such as graphene and carbon nanotubes (CNTs), that is, single-walled and multiwalled CNTs, against pheochromocytoma-derived PC-12 neural cells was also evaluated by quantifying metabolic activity (with WST-8 assay), intracellular oxidative stress (with ROS assay), and membrane integrity (with LDH assay).
Graphene films were grown by using chemical vapor deposition and were then coated onto glass coverslips by using the scooping method.
Graphene sheets were patterned on SiO2/Si substrates by using photolithography and were then covered with serum for a neural cell culture.
Both types of CNTs induced significant dose-dependent decreases in the viability of PC-12 cells, whereas graphene exerted adverse effects on the neural cells just at over 62.5 ppm.
This result implies that graphene and CNTs, even though they were the same carbon-based nanomaterials, show differential influences on neural cells.
Furthermore, graphene-coated or graphene-patterned substrates were shown to substantially enhance the adhesion and neurite outgrowth of PC-12 cells.
These results suggest that graphene-based substrates as biomimetic cues have good biocompatibility as well as a unique surface property that can enhance the neural cells, which would open up enormous opportunities in neural regeneration and nanomedicine.
American Psychological Association (APA)
Hong, Suck Won& Lee, Jong Ho& Kang, Seok Hee& Hwang, Eun Young& Hwang, Yu-Shik& Lee, Mi Hee…[et al.]. 2014. Enhanced Neural Cell Adhesion and Neurite Outgrowth on Graphene-Based Biomimetic Substrates. BioMed Research International،Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-454906
Modern Language Association (MLA)
Hong, Suck Won…[et al.]. Enhanced Neural Cell Adhesion and Neurite Outgrowth on Graphene-Based Biomimetic Substrates. BioMed Research International No. 2014 (2014), pp.1-8.
https://search.emarefa.net/detail/BIM-454906
American Medical Association (AMA)
Hong, Suck Won& Lee, Jong Ho& Kang, Seok Hee& Hwang, Eun Young& Hwang, Yu-Shik& Lee, Mi Hee…[et al.]. Enhanced Neural Cell Adhesion and Neurite Outgrowth on Graphene-Based Biomimetic Substrates. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-454906
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-454906