Treatment with at Homeopathic Complex Medication Modulates Mononuclear Bone Marrow Cell Differentiation
Joint Authors
Oliveira, Carolina Camargo
Gabardo, Juarez
Guimarães, Fernando S. F.
Cardoso, Francolino
de Freitas Buchi, Dorly
Abud, Ana Paula R.
Cesar, Beatriz
Bernardi, Raffaello Popa Di
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-02-17
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
A homeopathic complex medication (HCM), with immunomodulatory properties, is recommended for patients with depressed immune systems.
Previous studies demonstrated that the medication induces an increase in leukocyte number.
The bone marrow microenvironment is composed of growth factors, stromal cells, an extracellular matrix and progenitor cells that differentiate into mature blood cells.
Mice were our biological model used in this research.
We now report in vivo immunophenotyping of total bone marrow cells and ex vivo effects of the medication on mononuclear cell differentiation at different times.
Cells were examined by light microscopy and cytokine levels were measured in vitro.
After in vivo treatment with HCM, a pool of cells from the new marrow microenvironment was analyzed by flow cytometry to detect any trend in cell alteration.
The results showed decreases, mainly, in CD11b and TER-119 markers compared with controls.
Mononuclear cells were used to analyze the effects of ex vivo HCM treatment and the number of cells showing ring nuclei, niche cells and activated macrophages increased in culture, even in the absence of macrophage colony-stimulating factor.
Cytokines favoring stromal cell survival and differentiation in culture were induced in vitro.
Thus, we observe that HCM is immunomodulatory, either alone or in association with other products.
American Psychological Association (APA)
Cesar, Beatriz& Abud, Ana Paula R.& Oliveira, Carolina Camargo& Cardoso, Francolino& Bernardi, Raffaello Popa Di& Guimarães, Fernando S. F.…[et al.]. 2011. Treatment with at Homeopathic Complex Medication Modulates Mononuclear Bone Marrow Cell Differentiation. Evidence-Based Complementary and Alternative Medicine،Vol. 2011, no. 2011, pp.1-10.
https://search.emarefa.net/detail/BIM-454924
Modern Language Association (MLA)
Cesar, Beatriz…[et al.]. Treatment with at Homeopathic Complex Medication Modulates Mononuclear Bone Marrow Cell Differentiation. Evidence-Based Complementary and Alternative Medicine No. 2011 (2011), pp.1-10.
https://search.emarefa.net/detail/BIM-454924
American Medical Association (AMA)
Cesar, Beatriz& Abud, Ana Paula R.& Oliveira, Carolina Camargo& Cardoso, Francolino& Bernardi, Raffaello Popa Di& Guimarães, Fernando S. F.…[et al.]. Treatment with at Homeopathic Complex Medication Modulates Mononuclear Bone Marrow Cell Differentiation. Evidence-Based Complementary and Alternative Medicine. 2011. Vol. 2011, no. 2011, pp.1-10.
https://search.emarefa.net/detail/BIM-454924
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-454924