![](/images/graphics-bg.png)
Effects of Salvianolic Acid B on Protein Expression in Human Umbilical Vein Endothelial Cells
Joint Authors
Wang, Hui-Lin
Chang, Tsong-Min
Shi, Guey-Yueh
Su, Yan-Di
Wen, Hsin-Yun
Wu, Chieh-Hsi
Wu, Hua-Lin
Huang, Huey-Chun
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-03-02
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Salvianolic acid B (Sal B), a pure water-soluble compound extracted from Radix Salviae miltiorrhizae, has been reported to possess potential cardioprotective efficacy.
To identify proteins or pathways by which Sal B might exert its protective activities on the cardiovascular system, two-dimensional gel electrophoresis-based comparative proteomics was performed, and proteins altered in their expression level after Sal B treatment were identified by MALDI-TOF MS/MS.
Human umbilical vein endothelial cells (HUVECs) were incubated at Sal B concentrations that can be reached in human plasma by pharmacological intervention.
Results indicated that caldesmon, an actin-stabilizing protein, was downregulated in Sal B-exposed HUVECs.
Proteins that showed increased expression levels upon Sal B treatment were vimentin, T-complex protein 1, protein disulfide isomerase, tropomyosin alpha, heat shock protein beta-1, UBX domain-containing protein 1, alpha enolase, and peroxiredoxin-2.
Additionally, Sal B leads to increased phosphorylation of nucleophosmin in a dose-dependent manner and promotes proliferation of HUVECs.
We found that Sal B exhibited a coordinated regulation of enzymes and proteins involved in cytoskeletal reorganization, oxidative stress, and cell growth.
Our investigation would provide understanding to the endothelium protection information of Sal B.
American Psychological Association (APA)
Chang, Tsong-Min& Shi, Guey-Yueh& Wu, Hua-Lin& Wu, Chieh-Hsi& Su, Yan-Di& Wang, Hui-Lin…[et al.]. 2011. Effects of Salvianolic Acid B on Protein Expression in Human Umbilical Vein Endothelial Cells. Evidence-Based Complementary and Alternative Medicine،Vol. 2011, no. 2011, pp.1-9.
https://search.emarefa.net/detail/BIM-454979
Modern Language Association (MLA)
Chang, Tsong-Min…[et al.]. Effects of Salvianolic Acid B on Protein Expression in Human Umbilical Vein Endothelial Cells. Evidence-Based Complementary and Alternative Medicine No. 2011 (2011), pp.1-9.
https://search.emarefa.net/detail/BIM-454979
American Medical Association (AMA)
Chang, Tsong-Min& Shi, Guey-Yueh& Wu, Hua-Lin& Wu, Chieh-Hsi& Su, Yan-Di& Wang, Hui-Lin…[et al.]. Effects of Salvianolic Acid B on Protein Expression in Human Umbilical Vein Endothelial Cells. Evidence-Based Complementary and Alternative Medicine. 2011. Vol. 2011, no. 2011, pp.1-9.
https://search.emarefa.net/detail/BIM-454979
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-454979