Porous Materials to Support Bilayer Lipid Membranes for Ion Channel Biosensors
Joint Authors
Dunlop, James
Phung, Thai
Dalziel, Julie E.
Zhang, Yanli
Source
International Journal of Electrochemistry
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-08-29
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
To identify materials suitable as membrane supports for ion channel biosensors, six filter materials of varying hydrophobicity, tortuosity, and thickness were examined for their ability to support bilayer lipid membranes as determined by electrical impedance spectroscopy.
Bilayers supported by hydrophobic materials (PTFE, polycarbonate, nylon, and silanised silver) had optimal resistance (14–19 GΩ) and capacitance (0.8–1.6 μF) values whereas those with low hydrophobicity did not form BLMs (PVDF) or were short-lived (unsilanised silver).
The ability of ion channels to function in BLMs was assessed using a method recently reported to improve the efficiency of proteoliposome incorporation into PTFE-supported bilayers.
Voltage-gated sodium channel activation by veratridine and inhibition by saxitoxin showed activity for PTFE, nylon, and silanised silver, but not polycarbonate.
Bilayers on thicker, more tortuous, and hydrophobic materials produced higher current levels.
Bilayers that self-assembled on PTFE filters were the longest lived and produced the most channel activity using this method.
American Psychological Association (APA)
Phung, Thai& Zhang, Yanli& Dunlop, James& Dalziel, Julie E.. 2011. Porous Materials to Support Bilayer Lipid Membranes for Ion Channel Biosensors. International Journal of Electrochemistry،Vol. 2011, no. 2011, pp.1-6.
https://search.emarefa.net/detail/BIM-454985
Modern Language Association (MLA)
Phung, Thai…[et al.]. Porous Materials to Support Bilayer Lipid Membranes for Ion Channel Biosensors. International Journal of Electrochemistry No. 2011 (2011), pp.1-6.
https://search.emarefa.net/detail/BIM-454985
American Medical Association (AMA)
Phung, Thai& Zhang, Yanli& Dunlop, James& Dalziel, Julie E.. Porous Materials to Support Bilayer Lipid Membranes for Ion Channel Biosensors. International Journal of Electrochemistry. 2011. Vol. 2011, no. 2011, pp.1-6.
https://search.emarefa.net/detail/BIM-454985
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-454985