RSC96 Schwann Cell Proliferation and Survival Induced by Dilong through PI3KAkt Signaling Mediated by IGF-I
Joint Authors
Shih, Ying-Ting
Chang, Yung-Ming
Huang, Chih-Yang
Shu, Wen-Tong
Lai, Tung-Yuan
Tsai, Fuu-Jen
Chen, Yueh-Sheng
Kuo, Wei-Wen
Tsai, Chang-Hai
Chen, Ying-Yu
Kuo, Wu-Hsien
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-06-08
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Schwann cell proliferation is critical for the regeneration of injured nerves.
Dilongs are widely used in Chinese herbal medicine to remove stasis and stimulate wound-healing functions.
Exactly how this Chinese herbal medicine promotes tissue survival remains unclear.
The aim of the present study was to investigate the molecular mechanisms by which Dilong promote neuron regeneration.
Our results show that treatment with extract of Dilong induces the phosphorylation of the insulin-like growth factor-I (IGF-I)-mediated phosphatidylinositol 3-kinase/serine-threonine kinase (PI3K/Akt) pathway, and activates protein expression of cell nuclear antigen (PCNA) in a time-dependent manner.
Cell cycle analysis showed that G1 transits into the S phase in 12–16 h, and S transits into the G2 phase 20 h after exposure to earthworm extract.
Strong expression of cyclin D1, cyclin E and cyclin A occurs in a time-dependent manner.
Small interfering RNA (siRNA)-mediated knockdown of PI3K significantly reduced PI3K protein expression levels, resulting in Bcl2 survival factor reduction and a marked blockage of G1 to S transition in proliferating cells.
These results demonstrate that Dilong promotes the proliferation and survival of RSC96 cells via IGF-I signaling.
The mechanism is mainly dependent on the PI3K protein.
American Psychological Association (APA)
Chang, Yung-Ming& Kuo, Wu-Hsien& Lai, Tung-Yuan& Shih, Ying-Ting& Tsai, Fuu-Jen& Tsai, Chang-Hai…[et al.]. 2011. RSC96 Schwann Cell Proliferation and Survival Induced by Dilong through PI3KAkt Signaling Mediated by IGF-I. Evidence-Based Complementary and Alternative Medicine،Vol. 2011, no. 2011, pp.1-9.
https://search.emarefa.net/detail/BIM-455277
Modern Language Association (MLA)
Chang, Yung-Ming…[et al.]. RSC96 Schwann Cell Proliferation and Survival Induced by Dilong through PI3KAkt Signaling Mediated by IGF-I. Evidence-Based Complementary and Alternative Medicine No. 2011 (2011), pp.1-9.
https://search.emarefa.net/detail/BIM-455277
American Medical Association (AMA)
Chang, Yung-Ming& Kuo, Wu-Hsien& Lai, Tung-Yuan& Shih, Ying-Ting& Tsai, Fuu-Jen& Tsai, Chang-Hai…[et al.]. RSC96 Schwann Cell Proliferation and Survival Induced by Dilong through PI3KAkt Signaling Mediated by IGF-I. Evidence-Based Complementary and Alternative Medicine. 2011. Vol. 2011, no. 2011, pp.1-9.
https://search.emarefa.net/detail/BIM-455277
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-455277