Concurrent Dual Contrast for Cellular Magnetic Resonance Imaging Using Gadolinium Oxide and Iron Oxide Nanoparticles
Joint Authors
Loai, Yasir
Ganesh, Tameshwar
Margaret Cheng, Hai-Ling
Source
International Journal of Molecular Imaging
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-08-02
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Rationale and Objectives.
Concurrent visualization of differential targets in cellular and molecular imaging is valuable for resolving processes spatially and temporally, as in monitoring different cell subtypes.
The purpose of this study was to demonstrate concurrent, dual (positive and negative) contrast visualization on magnetic resonance imaging (MRI) of two colocalized cell populations labeled with Gadolinium “Gd” oxide and iron “Fe” oxide nanoparticles.
Materials and Methods.
Human aortic endothelial cells (EC) and smooth muscle cells (SMC) were labeled with various concentrations of Gd oxide and Fe oxide, respectively.
MRI on single- or mixed-cell samples was performed at 7 tesla.
Proper cell phenotype expressions, cell uptake of contrast agents, and the effect of labeling on cell viability and proliferation were also determined.
Results.
Both contrast agents were efficiently taken up by cells, with viability and proliferation largely unaffected.
On MRI, the positive contrast associated with Gd oxide-labeled EC and negative contrast associated with Fe oxide-labeled SMC discriminated the presence of each cell type, whether it existed alone or colocalized in a mixed-cell sample.
Conclusion.
It is feasible to use Gd oxide and Fe oxide for dual contrast and concurrent discrimination of two colocalized cell populations on MRI at 7 tesla.
American Psychological Association (APA)
Loai, Yasir& Ganesh, Tameshwar& Margaret Cheng, Hai-Ling. 2012. Concurrent Dual Contrast for Cellular Magnetic Resonance Imaging Using Gadolinium Oxide and Iron Oxide Nanoparticles. International Journal of Molecular Imaging،Vol. 2012, no. 2012, pp.1-10.
https://search.emarefa.net/detail/BIM-455749
Modern Language Association (MLA)
Loai, Yasir…[et al.]. Concurrent Dual Contrast for Cellular Magnetic Resonance Imaging Using Gadolinium Oxide and Iron Oxide Nanoparticles. International Journal of Molecular Imaging No. 2012 (2012), pp.1-10.
https://search.emarefa.net/detail/BIM-455749
American Medical Association (AMA)
Loai, Yasir& Ganesh, Tameshwar& Margaret Cheng, Hai-Ling. Concurrent Dual Contrast for Cellular Magnetic Resonance Imaging Using Gadolinium Oxide and Iron Oxide Nanoparticles. International Journal of Molecular Imaging. 2012. Vol. 2012, no. 2012, pp.1-10.
https://search.emarefa.net/detail/BIM-455749
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-455749