Cathepsin D Expression in Colorectal Cancer : From Proteomic Discovery through Validation Using Western Blotting, Immunohistochemistry, and Tissue Microarrays
Joint Authors
Bethwaite, Peter
Miller, Rose
Hayes, Mark
Jordan, T. William
Shi, Hongjun
Keating, John
Hood, Kylie
Kirana, Chandra
Stubbs, Richard S.
Laing, Emma
Source
International Journal of Proteomics
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-08-07
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Biology
Abstract EN
Despite recent advances in surgical techniques and therapeutic treatments, survival from colorectal cancer (CRC) remains disappointing with some 40–50% of newly diagnosed patients ultimately dying of metastatic disease.
Current staging by light microscopy alone is not sufficiently predictive of prognosis and would benefit from additional support from biomarkers in order to stratify patients appropriately for adjuvant therapy.
We have identified that cathepsin D expression was significantly greater in cells from invasive front (IF) area and liver metastasis (LM) than those from main tumour body (MTB).
Cathepsin D expression was subsequently examined by immunohistochemistry in tissue microarrays from 119 patients with CRC.
Strong expression in tumour cells at the IF did not correlate significantly with any clinico-pathological parameters examined or patient survival.
However, cathepsin D expression in cells from the MTB was highly elevated in late stage CRC and showed significant correlation with subsequent distant metastasis and shorter cancer-specific survival.
We also found that macrophages surrounding tumour cells stained strongly for cathepsin D but there was no significant correlation found between cathepsin D in macrophages at IF and MTB of CRC patient with the clinic-pathological parameters examined.
American Psychological Association (APA)
Kirana, Chandra& Shi, Hongjun& Laing, Emma& Hood, Kylie& Miller, Rose& Bethwaite, Peter…[et al.]. 2012. Cathepsin D Expression in Colorectal Cancer : From Proteomic Discovery through Validation Using Western Blotting, Immunohistochemistry, and Tissue Microarrays. International Journal of Proteomics،Vol. 2012, no. 2012, pp.1-10.
https://search.emarefa.net/detail/BIM-456942
Modern Language Association (MLA)
Kirana, Chandra…[et al.]. Cathepsin D Expression in Colorectal Cancer : From Proteomic Discovery through Validation Using Western Blotting, Immunohistochemistry, and Tissue Microarrays. International Journal of Proteomics No. 2012 (2012), pp.1-10.
https://search.emarefa.net/detail/BIM-456942
American Medical Association (AMA)
Kirana, Chandra& Shi, Hongjun& Laing, Emma& Hood, Kylie& Miller, Rose& Bethwaite, Peter…[et al.]. Cathepsin D Expression in Colorectal Cancer : From Proteomic Discovery through Validation Using Western Blotting, Immunohistochemistry, and Tissue Microarrays. International Journal of Proteomics. 2012. Vol. 2012, no. 2012, pp.1-10.
https://search.emarefa.net/detail/BIM-456942
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-456942