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Neutrophil Inhibitory Factor Selectively Inhibits the Endothelium-Driven Transmigration of Eosinophils In Vitro and Airway Eosinophilia in OVA-Induced Allergic Lung Inflammation
Joint Authors
Schnyder, Bruno
Moser, René
Schnyder-Candrian, Silvia
Maillet, Isabelle
Ryffel, Bernhard
Jacobs, Muazzam
Le Bert, Marc
Brault, Lea
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-12-04
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Leukocyte adhesion molecules are involved in cell recruitment in an allergic airway response and therefore provide a target for pharmaceutical intervention.
Neutrophil inhibitory factor (NIF), derived from canine hookworm (Ancylostoma caninum), binds selectively and competes with the A-domain of CD11b for binding to ICAM-1.
The effect of recombinant NIF was investigated.
Intranasal administration of rNIF reduced pulmonary eosinophilic infiltration, goblet cell hyperplasia, and Th2 cytokine production in OVA-sensitized mice.
In vitro, transendothelial migration of human blood eosinophils across IL-4-activated umbilical vein endothelial cell (HUVEC) monolayers was inhibited by rNIF (IC50: 4.6±2.6 nM; mean ± SEM), but not across TNF or IL-1-activated HUVEC monolayers.
Treatment of eosinophils with rNIF together with mAb 60.1 directed against CD11b or mAb 107 directed against the metal ion-dependent adhesion site (MIDAS) of the CD11b A-domain resulted in no further inhibition of transendothelial migration suggesting shared functional epitopes.
In contrast, rNIF increased the inhibitory effect of blocking mAbs against CD18, CD11a, and VLA-4.
Together, we show that rNIF, a selective antagonist of the A-domain of CD11b, has a prominent inhibitory effect on eosinophil transendothelial migration in vitro, which is congruent to the in vivo inhibition of OVA-induced allergic lung inflammation.
American Psychological Association (APA)
Schnyder-Candrian, Silvia& Maillet, Isabelle& Le Bert, Marc& Brault, Lea& Jacobs, Muazzam& Ryffel, Bernhard…[et al.]. 2012. Neutrophil Inhibitory Factor Selectively Inhibits the Endothelium-Driven Transmigration of Eosinophils In Vitro and Airway Eosinophilia in OVA-Induced Allergic Lung Inflammation. Journal of Allergy،Vol. 2012, no. 2012, pp.1-10.
https://search.emarefa.net/detail/BIM-456955
Modern Language Association (MLA)
Schnyder-Candrian, Silvia…[et al.]. Neutrophil Inhibitory Factor Selectively Inhibits the Endothelium-Driven Transmigration of Eosinophils In Vitro and Airway Eosinophilia in OVA-Induced Allergic Lung Inflammation. Journal of Allergy No. 2012 (2012), pp.1-10.
https://search.emarefa.net/detail/BIM-456955
American Medical Association (AMA)
Schnyder-Candrian, Silvia& Maillet, Isabelle& Le Bert, Marc& Brault, Lea& Jacobs, Muazzam& Ryffel, Bernhard…[et al.]. Neutrophil Inhibitory Factor Selectively Inhibits the Endothelium-Driven Transmigration of Eosinophils In Vitro and Airway Eosinophilia in OVA-Induced Allergic Lung Inflammation. Journal of Allergy. 2012. Vol. 2012, no. 2012, pp.1-10.
https://search.emarefa.net/detail/BIM-456955
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-456955