Influence of Egr-1 in Cardiac Tissue-Derived Mesenchymal Stem Cells in Response to Glucose Variations
Joint Authors
Mangino, Giorgio
De Falco, Elena
Coccia, Andrea
Pompilio, Giulio
Bastianelli, Daniela
Calogero, Antonella
Puca, Rosa
Bordin, Antonella
Siciliano, Camilla
Murdoch, Colin
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-05-22
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Mesenchymal stem cells (MSCs) represent a promising cell population for cell therapy and regenerative medicine applications.
However, how variations in glucose are perceived by MSC pool is still unclear.
Since, glucose metabolism is cell type and tissue dependent, this must be considered when MSCs are derived from alternative sources such as the heart.
The zinc finger transcription factor Egr-1 is an important early response gene, likely to play a key role in the glucose-induced response.
Our aim was to investigate how short-term changes in in vitro glucose concentrations affect multipotent cardiac tissue-derived MSCs (cMSCs) in a mouse model of Egr-1 KO (Egr-1−/−).
Results showed that loss of Egr-1 does not significantly influence cMSC proliferation.
In contrast, responses to glucose variations were observed in wt but not in Egr-1−/− cMSCs by clonogenic assay.
Phenotype analysis by RT-PCR showed that cMSCs Egr-1−/− lost the ability to regulate the glucose transporters GLUT-1 and GLUT-4 and, as expected, the Egr-1 target genes VEGF, TGFβ-1, and p300.
Acetylated protein levels of H3 histone were impaired in Egr-1−/− compared to wt cMSCs.
We propose that Egr-1 acts as immediate glucose biological sensor in cMSCs after a short period of stimuli, likely inducing epigenetic modifications.
American Psychological Association (APA)
Bastianelli, Daniela& Siciliano, Camilla& Puca, Rosa& Coccia, Andrea& Murdoch, Colin& Bordin, Antonella…[et al.]. 2014. Influence of Egr-1 in Cardiac Tissue-Derived Mesenchymal Stem Cells in Response to Glucose Variations. BioMed Research International،Vol. 2014, no. 2014, pp.1-11.
https://search.emarefa.net/detail/BIM-457795
Modern Language Association (MLA)
Bastianelli, Daniela…[et al.]. Influence of Egr-1 in Cardiac Tissue-Derived Mesenchymal Stem Cells in Response to Glucose Variations. BioMed Research International No. 2014 (2014), pp.1-11.
https://search.emarefa.net/detail/BIM-457795
American Medical Association (AMA)
Bastianelli, Daniela& Siciliano, Camilla& Puca, Rosa& Coccia, Andrea& Murdoch, Colin& Bordin, Antonella…[et al.]. Influence of Egr-1 in Cardiac Tissue-Derived Mesenchymal Stem Cells in Response to Glucose Variations. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-11.
https://search.emarefa.net/detail/BIM-457795
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-457795