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New Spectrophotometric and Fluorimetric Methods for Determination of Fluoxetine in Pharmaceutical Formulations
Joint Authors
Darwish, Ibrahim A.
Abdine, Heba H.
Amer, Sawsan M.
al-Rayes, Lama I.
Source
International Journal of Analytical Chemistry
Issue
Vol. 2009, Issue 2009 (31 Dec. 2009), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2009-08-16
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
New simple and sensitive spectrophotometric and fluorimetric methods have been developed and validated for the determination of fluoxetine hydrochloride (FLX) in its pharmaceutical formulations.
The spectrophotometric method was based on the reaction of FLX with 1,2-naphthoquinone-4-sulphonate (NQS) in an alkaline medium (pH 11) to form an orange-colored product that was measured at 490 nm.
The fluorimetric method was based on the reaction of FLX with 4-chloro-7-nitrobenzo-2-oxa-1,3-diazole (NBD-Cl) in an alkaline medium (pH 8) to form a highly fluorescent product that was measured at 545 nm after excitation at 490 nm.
The variables affecting the reactions of FLX with both NQS and NBD-Cl were carefully studied and optimized.
The kinetics of the reactions were investigated, and the reaction mechanisms were presented.
Under the optimum reaction conditions, good linear relationships were found between the readings and the concentrations of FLX in the ranges of 0.3–6 and 0.035–0.5 μg mL−1 for the spectrophotometric and fluorimetric methods, respectively.
The limits of detection were 0.1 and 0.01 μg mL−1 for the spectrophotometric and fluorimetric methods, respectively.
Both methods were successfully applied to the determination of FLX in its pharmaceutical formulations.
American Psychological Association (APA)
Darwish, Ibrahim A.& Amer, Sawsan M.& Abdine, Heba H.& al-Rayes, Lama I.. 2009. New Spectrophotometric and Fluorimetric Methods for Determination of Fluoxetine in Pharmaceutical Formulations. International Journal of Analytical Chemistry،Vol. 2009, no. 2009, pp.1-9.
https://search.emarefa.net/detail/BIM-457954
Modern Language Association (MLA)
Darwish, Ibrahim A.…[et al.]. New Spectrophotometric and Fluorimetric Methods for Determination of Fluoxetine in Pharmaceutical Formulations. International Journal of Analytical Chemistry No. 2009 (2009), pp.1-9.
https://search.emarefa.net/detail/BIM-457954
American Medical Association (AMA)
Darwish, Ibrahim A.& Amer, Sawsan M.& Abdine, Heba H.& al-Rayes, Lama I.. New Spectrophotometric and Fluorimetric Methods for Determination of Fluoxetine in Pharmaceutical Formulations. International Journal of Analytical Chemistry. 2009. Vol. 2009, no. 2009, pp.1-9.
https://search.emarefa.net/detail/BIM-457954
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-457954