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In Vivo Visualization of Heterogeneous Intratumoral Distribution of Hypoxia-Inducible Factor-1α Activity by the Fusion of High-Resolution SPECT and Morphological Imaging Tests
Joint Authors
Inoue, Kazumasa
Moriyama, Noriyuki
Yamaguchi, Masayuki
Mutou, Yasuko
Saji, Hideo
Fujii, Hirofumi
Ueda, Masashi
Kizaka-Kondoh, Shinae
Umeda, Izumi O.
Source
Journal of Biomedicine and Biotechnology
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-06-12
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
Purpose.
We aimed to clearly visualize heterogeneous distribution of hypoxia-inducible factor 1α (HIF) activity in tumor tissues in vivo.
Methods.
We synthesized of 125I-IPOS, a 125I labeled chimeric protein probe, that would visualize HIF activity.
The biodistribution of 125I-IPOS in FM3A tumor-bearing mice was evaluated.
Then, the intratumoral localization of this probe was observed by autoradiography, and it was compared with histopathological findings.
The distribution of 125I-IPOS in tumors was imaged by a small animal SPECT/CT scanner.
The obtained in vivo SPECT-CT fusion images were compared with ex vivo images of excised tumors.
Fusion imaging with MRI was also examined.
Results.
125I-IPOS well accumulated in FM3A tumors.
The intratumoral distribution of 125I-IPOS by autoradiography was quite heterogeneous, and it partially overlapped with that of pimonidazole.
High-resolution SPECT-CT fusion images successfully demonstrated the heterogeneity of 125I-IPOS distribution inside tumors.
SPECT-MRI fusion images could give more detailed information about the intratumoral distribution of 125I-IPOS.
Conclusion.
High-resolution SPECT images successfully demonstrated heterogeneous intratumoral distribution of 125I-IPOS.
SPECT-CT fusion images, more favorably SPECT-MRI fusion images, would be useful to understand the features of heterogeneous intratumoral expression of HIF activity in vivo.
American Psychological Association (APA)
Fujii, Hirofumi& Yamaguchi, Masayuki& Inoue, Kazumasa& Mutou, Yasuko& Ueda, Masashi& Saji, Hideo…[et al.]. 2012. In Vivo Visualization of Heterogeneous Intratumoral Distribution of Hypoxia-Inducible Factor-1α Activity by the Fusion of High-Resolution SPECT and Morphological Imaging Tests. Journal of Biomedicine and Biotechnology،Vol. 2012, no. 2012, pp.1-6.
https://search.emarefa.net/detail/BIM-458482
Modern Language Association (MLA)
Fujii, Hirofumi…[et al.]. In Vivo Visualization of Heterogeneous Intratumoral Distribution of Hypoxia-Inducible Factor-1α Activity by the Fusion of High-Resolution SPECT and Morphological Imaging Tests. Journal of Biomedicine and Biotechnology No. 2012 (2012), pp.1-6.
https://search.emarefa.net/detail/BIM-458482
American Medical Association (AMA)
Fujii, Hirofumi& Yamaguchi, Masayuki& Inoue, Kazumasa& Mutou, Yasuko& Ueda, Masashi& Saji, Hideo…[et al.]. In Vivo Visualization of Heterogeneous Intratumoral Distribution of Hypoxia-Inducible Factor-1α Activity by the Fusion of High-Resolution SPECT and Morphological Imaging Tests. Journal of Biomedicine and Biotechnology. 2012. Vol. 2012, no. 2012, pp.1-6.
https://search.emarefa.net/detail/BIM-458482
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-458482