High Potential Source for Biomass Degradation Enzyme Discovery and Environmental Aspects Revealed through Metagenomics of Indian Buffalo Rumen
Joint Authors
Joshi, C. G.
Singh, K. M.
Patel, J. B.
Reddy, Bhaskar
Patel, A. K.
Parmar, Nidhi
Patel, Dishita
Patel, Anand
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-07-17
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
The complex microbiomes of the rumen functions as an effective system for plant cell wall degradation, and biomass utilization provide genetic resource for degrading microbial enzymes that could be used in the production of biofuel.
Therefore the buffalo rumen microbiota was surveyed using shot gun sequencing.
This metagenomic sequencing generated 3.9 GB of sequences and data were assembled into 137270 contiguous sequences (contigs).
We identified potential 2614 contigs encoding biomass degrading enzymes including glycoside hydrolases (GH: 1943 contigs), carbohydrate binding module (CBM: 23 contigs), glycosyl transferase (GT: 373 contigs), carbohydrate esterases (CE: 259 contigs), and polysaccharide lyases (PE: 16 contigs).
The hierarchical clustering of buffalo metagenomes demonstrated the similarities and dissimilarity in microbial community structures and functional capacity.
This demonstrates that buffalo rumen microbiome was considerably enriched in functional genes involved in polysaccharide degradation with great prospects to obtain new molecules that may be applied in the biofuel industry.
American Psychological Association (APA)
Singh, K. M.& Reddy, Bhaskar& Patel, Dishita& Patel, A. K.& Parmar, Nidhi& Patel, Anand…[et al.]. 2014. High Potential Source for Biomass Degradation Enzyme Discovery and Environmental Aspects Revealed through Metagenomics of Indian Buffalo Rumen. BioMed Research International،Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-458801
Modern Language Association (MLA)
Singh, K. M.…[et al.]. High Potential Source for Biomass Degradation Enzyme Discovery and Environmental Aspects Revealed through Metagenomics of Indian Buffalo Rumen. BioMed Research International No. 2014 (2014), pp.1-10.
https://search.emarefa.net/detail/BIM-458801
American Medical Association (AMA)
Singh, K. M.& Reddy, Bhaskar& Patel, Dishita& Patel, A. K.& Parmar, Nidhi& Patel, Anand…[et al.]. High Potential Source for Biomass Degradation Enzyme Discovery and Environmental Aspects Revealed through Metagenomics of Indian Buffalo Rumen. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-458801
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-458801