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Assessment of Clinical Diagnosis, Microscopy, Rapid Diagnostic Tests, and Polymerase Chain Reaction in the Diagnosis of Plasmodium falciparum in Nigeria
Joint Authors
Olowe, Olugbenga Adekunle
Ojurongbe, Olusola
Taiwo, Sunday Samuel
Ojurongbe, Taiwo Adetola
Bolaji, Oloyede Samuel
Adegbosin, Olunike Olayeni
Adeyeba, Oluwaseyi Adegboyega
Alli, Oyebode Armstrong Terry
Source
Malaria Research and Treatment
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-5, 5 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-11-24
Country of Publication
Egypt
No. of Pages
5
Main Subjects
Abstract EN
This study compares the performance of clinical diagnosis and three laboratory diagnostic methods (thick film microscopy (TFM), rapid diagnostic test (RDT), and polymerase chain reaction (PCR)) for the diagnosis of Plasmodium falciparum in Nigeria.
Using clinical criteria, 217 children were recruited into the study out of which 106 (48.8%) were positive by TFM, 84 (38.7%) by RDT, and 125 (57.6%) by PCR.
Using a composite reference method generated from the three diagnostic methods, 71 (32.7%) patients were found to be truly infected and 90 (41.5%) truly uninfected, while 56 (25.8%) were misidentified as infected or noninfected.
When each of the 3 diagnostic methods was compared with the composite reference, PCR had sensitivity of 97.3%, specificity of 62.5%, positive predictive value (PPV) of 56.8%, and negative predictive value (NPV) of 97.8%; microscopy had sensitivity of 77.2%, specificity of 72%, PPV of 66.9%, and NPV of 81.1%, while RDT had sensitivity of 62.3%, specificity of 87.4%, PPV of 67.7%, and NPV of 84.5%.
PCR test performed best among the three methods followed by TFM and RDT in that order.
The result of this study shows that clinical diagnosis cannot be relied upon for accurate diagnosis of P.
falciparum in endemic areas.
American Psychological Association (APA)
Ojurongbe, Olusola& Adegbosin, Olunike Olayeni& Taiwo, Sunday Samuel& Alli, Oyebode Armstrong Terry& Olowe, Olugbenga Adekunle& Ojurongbe, Taiwo Adetola…[et al.]. 2013. Assessment of Clinical Diagnosis, Microscopy, Rapid Diagnostic Tests, and Polymerase Chain Reaction in the Diagnosis of Plasmodium falciparum in Nigeria. Malaria Research and Treatment،Vol. 2013, no. 2013, pp.1-5.
https://search.emarefa.net/detail/BIM-462221
Modern Language Association (MLA)
Ojurongbe, Olusola…[et al.]. Assessment of Clinical Diagnosis, Microscopy, Rapid Diagnostic Tests, and Polymerase Chain Reaction in the Diagnosis of Plasmodium falciparum in Nigeria. Malaria Research and Treatment No. 2013 (2013), pp.1-5.
https://search.emarefa.net/detail/BIM-462221
American Medical Association (AMA)
Ojurongbe, Olusola& Adegbosin, Olunike Olayeni& Taiwo, Sunday Samuel& Alli, Oyebode Armstrong Terry& Olowe, Olugbenga Adekunle& Ojurongbe, Taiwo Adetola…[et al.]. Assessment of Clinical Diagnosis, Microscopy, Rapid Diagnostic Tests, and Polymerase Chain Reaction in the Diagnosis of Plasmodium falciparum in Nigeria. Malaria Research and Treatment. 2013. Vol. 2013, no. 2013, pp.1-5.
https://search.emarefa.net/detail/BIM-462221
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-462221