Sildenafil (Viagra) Protective Effects on Neuroinflammation : The Role of iNOSNO System in an Inflammatory Demyelination Model
Joint Authors
Raposo, Catarina
Luna, Rayana Leal de Almeida
Peixoto, Christina Alves
da Cruz-Höfling, Maria Alice
Araújo, Shyrlene Meiry da Rocha
Nunes, Ana Karolina de Santana
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-07-22
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
We recently demonstrated that sildenafil reduces the expression of cytokines, COX-2, and GFAP in a demyelinating model induced in wild-type (WT) mice.
Herein, the understandings of the neuroprotective effect of sildenafil and the mediation of iNOS/NO system on inflammatory demyelination induced by cuprizone were investigated.
The cerebella of iNOS−/− mice were examined after four weeks of treatment with cuprizone alone or combined with sildenafil.
Cuprizone increased GFAP, Iba-1, TNF-α, COX-2, IL-1β, and IFN-γ expression, decreased expression of glutathione S-transferase pi (GSTpi), and damaged myelin in iNOS−/− mice.
Sildenafil reduced Iba-1, IFN-γ, and IL-1β levels but had no effect on the expression of GFAP, TNF-α, and COX-2 compared to the cuprizone group.
Sildenafil elevated GSTpi levels and improved the myelin structure/ultrastructure.
iNOS−/− mice suffered from severe inflammation following treatment with cuprizone, while WT mice had milder inflammation, as found in the previous study.
It is possible that inflammatory regulation through iNOS-feedback is absent in iNOS−/− mice, making them more susceptible to inflammation.
Sildenafil has at least a partial anti-inflammatory effect through iNOS inhibition, as its effect on iNOS−/− mice was limited.
Further studies are required to explain the underlying mechanism of the sildenafil effects.
American Psychological Association (APA)
Raposo, Catarina& Nunes, Ana Karolina de Santana& Luna, Rayana Leal de Almeida& Araújo, Shyrlene Meiry da Rocha& da Cruz-Höfling, Maria Alice& Peixoto, Christina Alves. 2013. Sildenafil (Viagra) Protective Effects on Neuroinflammation : The Role of iNOSNO System in an Inflammatory Demyelination Model. Mediators of Inflammation،Vol. 2013, no. 2013, pp.1-11.
https://search.emarefa.net/detail/BIM-463365
Modern Language Association (MLA)
Raposo, Catarina…[et al.]. Sildenafil (Viagra) Protective Effects on Neuroinflammation : The Role of iNOSNO System in an Inflammatory Demyelination Model. Mediators of Inflammation No. 2013 (2013), pp.1-11.
https://search.emarefa.net/detail/BIM-463365
American Medical Association (AMA)
Raposo, Catarina& Nunes, Ana Karolina de Santana& Luna, Rayana Leal de Almeida& Araújo, Shyrlene Meiry da Rocha& da Cruz-Höfling, Maria Alice& Peixoto, Christina Alves. Sildenafil (Viagra) Protective Effects on Neuroinflammation : The Role of iNOSNO System in an Inflammatory Demyelination Model. Mediators of Inflammation. 2013. Vol. 2013, no. 2013, pp.1-11.
https://search.emarefa.net/detail/BIM-463365
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-463365