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Image Processing Techniques for Assessing Contractility in Isolated Adult Cardiac Myocytes
Joint Authors
Bazan, Carlos
Barba, David Torres
Paolini, Paul
Blomgren, Peter
Source
International Journal of Biomedical Imaging
Issue
Vol. 2009, Issue 2009 (31 Dec. 2009), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-02-24
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
We describe a computational framework for the comprehensive assessment of contractile responses of enzymatically dissociated adult cardiac myocytes.
The proposed methodology comprises the following stages: digital video recording of the contracting cell, edge preserving total variation-based image smoothing, segmentation of the smoothed images, contour extraction from the segmented images, shape representation by Fourier descriptors, and contractility assessment.
The different stages are variants of mathematically sound and computationally robust algorithms very well established in the image processing community.
The physiologic application of the methodology is evaluated by assessing overall contraction in enzymatically dissociated adult rat cardiocytes.
Our results demonstrate the effectiveness of the proposed approach in characterizing the true, two-dimensional, “shortening” in the contraction process of adult cardiocytes.
We compare the performance of the proposed method to that of a popular edge detection system in the literature.
The proposed method not only provides a more comprehensive assessment of the myocyte contraction process but also can potentially eliminate historical concerns and sources of errors caused by myocyte rotation or translation during contraction.
Furthermore, the versatility of the image processing techniques makes the method suitable for determining myocyte shortening in cells that usually bend or move during contraction.
The proposed method can be utilized to evaluate changes in contractile behavior resulting from drug intervention, disease modeling, transgeneity, or other common applications to mammalian cardiocytes.
American Psychological Association (APA)
Bazan, Carlos& Barba, David Torres& Blomgren, Peter& Paolini, Paul. 2010. Image Processing Techniques for Assessing Contractility in Isolated Adult Cardiac Myocytes. International Journal of Biomedical Imaging،Vol. 2009, no. 2009, pp.1-11.
https://search.emarefa.net/detail/BIM-465186
Modern Language Association (MLA)
Bazan, Carlos…[et al.]. Image Processing Techniques for Assessing Contractility in Isolated Adult Cardiac Myocytes. International Journal of Biomedical Imaging No. 2009 (2009), pp.1-11.
https://search.emarefa.net/detail/BIM-465186
American Medical Association (AMA)
Bazan, Carlos& Barba, David Torres& Blomgren, Peter& Paolini, Paul. Image Processing Techniques for Assessing Contractility in Isolated Adult Cardiac Myocytes. International Journal of Biomedical Imaging. 2010. Vol. 2009, no. 2009, pp.1-11.
https://search.emarefa.net/detail/BIM-465186
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-465186