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Differential Gene Expression in High- and Low-Active Inbred Mice
Joint Authors
Kochan, Kelli J.
Riggs, Penny K.
Ceaser, Tyrone
Dawes, Michelle
Lightfoot, J. Timothy
Hamilton, Alicia T.
Moore-Harrison, Trudy
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-01-16
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Numerous candidate genes have been suggested in the recent literature with proposed roles in regulation of voluntary physical activity, with little evidence of these genes’ functional roles.
This study compared the haplotype structure and expression profile in skeletal muscle and brain of inherently high- (C57L/J) and low- (C3H/HeJ) active mice.
Expression of nine candidate genes [Actn2, Actn3, Casq1, Drd2, Lepr, Mc4r, Mstn, Papss2, and Glut4 (a.k.a.
Slc2a4)] was evaluated via RT-qPCR.
SNPs were observed in regions of Actn2, Casq1, Drd2, Lepr, and Papss2; however, no SNPs were located in coding sequences or associated with any known regulatory sequences.
In mice exposed to a running wheel, Casq1 (P=0.0003) and Mstn (P=0.002) transcript levels in the soleus were higher in the low-active mice.
However, when these genes were evaluated in naïve animals, differential expression was not observed, demonstrating a training effect.
Among naïve mice, no genes in either tissue exhibited differential expression between strains.
Considering that no obvious SNP mechanisms were determined or differential expression was observed, our results indicate that genomic structural variation or gene expression data alone is not adequate to establish any of these genes’ candidacy or causality in relation to regulation of physical activity.
American Psychological Association (APA)
Dawes, Michelle& Moore-Harrison, Trudy& Hamilton, Alicia T.& Ceaser, Tyrone& Kochan, Kelli J.& Riggs, Penny K.…[et al.]. 2014. Differential Gene Expression in High- and Low-Active Inbred Mice. BioMed Research International،Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-465854
Modern Language Association (MLA)
Dawes, Michelle…[et al.]. Differential Gene Expression in High- and Low-Active Inbred Mice. BioMed Research International No. 2014 (2014), pp.1-9.
https://search.emarefa.net/detail/BIM-465854
American Medical Association (AMA)
Dawes, Michelle& Moore-Harrison, Trudy& Hamilton, Alicia T.& Ceaser, Tyrone& Kochan, Kelli J.& Riggs, Penny K.…[et al.]. Differential Gene Expression in High- and Low-Active Inbred Mice. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-465854
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-465854