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Unique Characteristics of the Pyrrolysine System in the 7th Order of Methanogens : Implications for the Evolution of a Genetic Code Expansion Cassette
Joint Authors
O'Toole, Paul W.
Peyret, Pierre
Brugère, Jean-François
Gribaldo, Simonetta
Borrel, Guillaume
Gaci, Nadia
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-01-27
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Pyrrolysine (Pyl), the 22nd proteogenic amino acid, was restricted until recently to few organisms.
Its translational use necessitates the presence of enzymes for synthesizing it from lysine, a dedicated amber stop codon suppressor tRNA, and a specific amino-acyl tRNA synthetase.
The three genomes of the recently proposed Thermoplasmata-related 7th order of methanogens contain the complete genetic set for Pyl synthesis and its translational use.
Here, we have analyzed the genomic features of the Pyl-coding system in these three genomes with those previously known from Bacteria and Archaea and analyzed the phylogeny of each component.
This shows unique peculiarities, notably an amber tRNAPyl with an imperfect anticodon stem and a shortened tRNAPyl synthetase.
Phylogenetic analysis indicates that a Pyl-coding system was present in the ancestor of the seventh order of methanogens and appears more closely related to Bacteria than to Methanosarcinaceae, suggesting the involvement of lateral gene transfer in the spreading of pyrrolysine between the two prokaryotic domains.
We propose that the Pyl-coding system likely emerged once in Archaea, in a hydrogenotrophic and methanol-H2-dependent methylotrophic methanogen.
The close relationship between methanogenesis and the Pyl system provides a possible example of expansion of a still evolving genetic code, shaped by metabolic requirements.
American Psychological Association (APA)
Borrel, Guillaume& Gaci, Nadia& Peyret, Pierre& O'Toole, Paul W.& Gribaldo, Simonetta& Brugère, Jean-François. 2014. Unique Characteristics of the Pyrrolysine System in the 7th Order of Methanogens : Implications for the Evolution of a Genetic Code Expansion Cassette. Archaea،Vol. 2014, no. 2014, pp.1-11.
https://search.emarefa.net/detail/BIM-467002
Modern Language Association (MLA)
Borrel, Guillaume…[et al.]. Unique Characteristics of the Pyrrolysine System in the 7th Order of Methanogens : Implications for the Evolution of a Genetic Code Expansion Cassette. Archaea No. 2014 (2014), pp.1-11.
https://search.emarefa.net/detail/BIM-467002
American Medical Association (AMA)
Borrel, Guillaume& Gaci, Nadia& Peyret, Pierre& O'Toole, Paul W.& Gribaldo, Simonetta& Brugère, Jean-François. Unique Characteristics of the Pyrrolysine System in the 7th Order of Methanogens : Implications for the Evolution of a Genetic Code Expansion Cassette. Archaea. 2014. Vol. 2014, no. 2014, pp.1-11.
https://search.emarefa.net/detail/BIM-467002
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-467002