Modified High-Sucrose Diet-Induced Abdominally Obese and Normal-Weight Rats Developed High Plasma Free Fatty Acid and Insulin Resistance
Joint Authors
Cao, Hongyi
Lv, Qingguo
Liu, Xuehui
Cao, Li
Tong, Nanwei
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-12-25
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Biology
Abstract EN
Introduction.
Metabolically obese but normal-weight (MONW) individuals have metabolic features of overt obesity, and abdominal adiposity is common in them.
Animal models of MONW individuals are lacking.
We aimed to develop an abdominally obese and normal-weight (AONW) rat model.
Methods and Results.
Young male Sprague-Dawley rats were fed chow or a modified high-sucrose (HS) diet for 20 weeks.
The HS diet induced increased visceral adipose tissue without increased body weight, reduced glucose disposal rates, and increased hepatic glucose output during the hyperinsulinemic-euglycemic clamp, increased plasma glucose during the intraperitoneal glucose tolerance test, and increased plasma free fatty acids.
Hepatic lipidosis and hepatocyte mitochondria swelling were found in HS rats through light microscopy and transmission electron microscopy; similar impairments were not observed in muscle.
RT-PCR showed that mRNA expression of uncoupling protein 3 and peroxisome proliferator-activated receptor-gamma coactivator 1α increased in muscle of HS rats, while expression of mitochondrial transcription factor A, glucose transporter type 4, and insulin receptor substrate-1 did not change significantly.
Conclusion.
AONW rats developed metabolic disorders seen in MONW individuals.
Steatosis, mitochondrial morphologic changes, and insulin resistance were more serious in liver than in muscle.
Genes involved in fatty acid metabolism and mitochondrial function changed in less impaired muscle.
American Psychological Association (APA)
Cao, Li& Liu, Xuehui& Cao, Hongyi& Lv, Qingguo& Tong, Nanwei. 2012. Modified High-Sucrose Diet-Induced Abdominally Obese and Normal-Weight Rats Developed High Plasma Free Fatty Acid and Insulin Resistance. Oxidative Medicine and Cellular Longevity،Vol. 2012, no. 2012, pp.1-9.
https://search.emarefa.net/detail/BIM-467025
Modern Language Association (MLA)
Cao, Li…[et al.]. Modified High-Sucrose Diet-Induced Abdominally Obese and Normal-Weight Rats Developed High Plasma Free Fatty Acid and Insulin Resistance. Oxidative Medicine and Cellular Longevity No. 2012 (2012), pp.1-9.
https://search.emarefa.net/detail/BIM-467025
American Medical Association (AMA)
Cao, Li& Liu, Xuehui& Cao, Hongyi& Lv, Qingguo& Tong, Nanwei. Modified High-Sucrose Diet-Induced Abdominally Obese and Normal-Weight Rats Developed High Plasma Free Fatty Acid and Insulin Resistance. Oxidative Medicine and Cellular Longevity. 2012. Vol. 2012, no. 2012, pp.1-9.
https://search.emarefa.net/detail/BIM-467025
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-467025