Endonuclease IV Is the Main Base Excision Repair Enzyme Involved in DNA Damage Induced by UVA Radiation and Stannous Chloride
Joint Authors
De Mattos, José Carlos P.
Dantas, Flávio J. S.
Caldeira-de-Araújo, Adriano
Motta, Ellen S.
Souza-Santos, Paulo Thiago
Cassiano, Tuany R.
Source
Journal of Biomedicine and Biotechnology
Issue
Vol. 2010, Issue 2010 (31 Dec. 2010), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-03-15
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Stannous chloride (SnCl2) and UVA induce DNA lesions through ROS.
The aim of this work was to study the toxicity induced by UVA preillumination, followed by SnCl2 treatment.
E.
coli BER mutants were used to identify genes which could play a role in DNA lesion repair generated by these agents.
The survival assays showed (i) The nfo mutant was the most sensitive to SnCl2; (ii) lethal synergistic effect was observed after UVA pre-illumination, plus SnCl2 incubation, the nfo mutant being the most sensitive; (iii) wild type and nfo mutants, transformed with pBW21 plasmid (nfo+) had their survival increased following treatments.
The alkaline agarose gel electrophoresis assays pointed that (i) UVA induced DNA breaks and fpg mutant was the most sensitive; (ii) SnCl2-induced DNA strand breaks were higher than those from UVA and nfo mutant had the slowest repair kinetics; (iii) UVA+SnCl2 promoted an increase in DNA breaks than SnCl2 and, again, nfo mutant displayed the slowest repair kinetics.
In summary, Nfo protects E.
coli cells against damage induced by SnCl2 and UVA+ SnCl2.
American Psychological Association (APA)
Motta, Ellen S.& Souza-Santos, Paulo Thiago& Cassiano, Tuany R.& Dantas, Flávio J. S.& Caldeira-de-Araújo, Adriano& De Mattos, José Carlos P.. 2010. Endonuclease IV Is the Main Base Excision Repair Enzyme Involved in DNA Damage Induced by UVA Radiation and Stannous Chloride. Journal of Biomedicine and Biotechnology،Vol. 2010, no. 2010, pp.1-9.
https://search.emarefa.net/detail/BIM-467218
Modern Language Association (MLA)
Motta, Ellen S.…[et al.]. Endonuclease IV Is the Main Base Excision Repair Enzyme Involved in DNA Damage Induced by UVA Radiation and Stannous Chloride. Journal of Biomedicine and Biotechnology No. 2010 (2010), pp.1-9.
https://search.emarefa.net/detail/BIM-467218
American Medical Association (AMA)
Motta, Ellen S.& Souza-Santos, Paulo Thiago& Cassiano, Tuany R.& Dantas, Flávio J. S.& Caldeira-de-Araújo, Adriano& De Mattos, José Carlos P.. Endonuclease IV Is the Main Base Excision Repair Enzyme Involved in DNA Damage Induced by UVA Radiation and Stannous Chloride. Journal of Biomedicine and Biotechnology. 2010. Vol. 2010, no. 2010, pp.1-9.
https://search.emarefa.net/detail/BIM-467218
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-467218