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Methylation-Associated Gene Silencing of RARB in Areca Carcinogens Induced Mouse Oral Squamous Cell Carcinoma
Joint Authors
Lai, Zi-Lun
Tsai, Ming-Hsui
Chang, Nai-Wen
Chen, Chuan-Mu
Fan, Shin-Ru
Cheng, Ju-Chien
Tsou, Yung-An
Chen, Hsiao-Ling
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-08-17
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
Regarding oral squamous cell carcinoma (OSCC) development, chewing areca is known to be a strong risk factor in many Asian cultures.
Therefore, we established an OSCC induced mouse model by 4-nitroquinoline-1-oxide (4-NQO), or arecoline, or both treatments, respectively.
These are the main two components of the areca nut that could increase the occurrence of OSCC.
We examined the effects with the noncommercial MCGI (mouse CpG islands) microarray for genome-wide screening the DNA methylation aberrant in induced OSCC mice.
The microarray results showed 34 hypermethylated genes in 4-NQO plus arecoline induced OSCC mice tongue tissues.
The examinations also used methylation-specific polymerase chain reaction (MS-PCR) and bisulfite sequencing to realize the methylation pattern in collected mouse tongue tissues and human OSCC cell lines of different grades, respectively.
These results showed that retinoic acid receptor β (RARB) was indicated in hypermethylation at the promoter region and the loss of expression during cancer development.
According to the results of real-time PCR, it was shown that de novo DNA methyltransferases were involved in gene epigenetic alternations of OSCC.
Collectively, our results showed that RARB hypermethylation was involved in the areca-associated oral carcinogenesis.
American Psychological Association (APA)
Lai, Zi-Lun& Tsou, Yung-An& Fan, Shin-Ru& Tsai, Ming-Hsui& Chen, Hsiao-Ling& Chang, Nai-Wen…[et al.]. 2014. Methylation-Associated Gene Silencing of RARB in Areca Carcinogens Induced Mouse Oral Squamous Cell Carcinoma. BioMed Research International،Vol. 2014, no. 2014, pp.1-13.
https://search.emarefa.net/detail/BIM-467309
Modern Language Association (MLA)
Lai, Zi-Lun…[et al.]. Methylation-Associated Gene Silencing of RARB in Areca Carcinogens Induced Mouse Oral Squamous Cell Carcinoma. BioMed Research International No. 2014 (2014), pp.1-13.
https://search.emarefa.net/detail/BIM-467309
American Medical Association (AMA)
Lai, Zi-Lun& Tsou, Yung-An& Fan, Shin-Ru& Tsai, Ming-Hsui& Chen, Hsiao-Ling& Chang, Nai-Wen…[et al.]. Methylation-Associated Gene Silencing of RARB in Areca Carcinogens Induced Mouse Oral Squamous Cell Carcinoma. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-13.
https://search.emarefa.net/detail/BIM-467309
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-467309