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Persea americana Mill. Seed : Fractionation, Characterization, and Effects on Human Keratinocytes and Fibroblasts
Joint Authors
Deters, Alexandra M.
Jerz, Gerold
Villanueva, Socorro
Ramos-Jerz, Maria del R.
Winterhalter, Peter
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-11-28
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Methanolic avocado (Persea americana Mill., Lauraceae) seed extracts were separated by preparative HSCCC.
Partition and HSCCC fractions were principally characterized by LC-ESI-MS/MS analysis.
Their in vitro influence was investigated on proliferation, differentiation, cell viability, and gene expression on HaCaT and normal human epidermal keratinocytes (NHEK) and normal human dermal fibroblasts (NHDF).
The methanol-water partition (M) from avocado seeds and HSCCC fraction 3 (M.3) were mostly composed of chlorogenic acid and its isomers.
Both reduced NHDF but enhanced HaCaT keratinocytes proliferation.
HSCCC fraction M.2 composed of quinic acid among chlorogenic acid and its isomers inhibited proliferation and directly induced differentiation of keratinocytes as observed on gene and protein level.
Furthermore, M.2 increased NHDF proliferation via upregulation of growth factor receptors.
Salidrosides and ABA derivatives present in HSCCC fraction M.6 increased NHDF and keratinocyte proliferation that resulted in differentiation.
The residual solvent fraction M.7 contained among low concentrations of ABA derivatives high amounts of proanthocyanidins B1 and B2 as well as an A-type trimer and stimulated proliferation of normal cells and inhibited the proliferation of immortalized HaCaT keratinocytes.
American Psychological Association (APA)
Ramos-Jerz, Maria del R.& Villanueva, Socorro& Jerz, Gerold& Winterhalter, Peter& Deters, Alexandra M.. 2013. Persea americana Mill. Seed : Fractionation, Characterization, and Effects on Human Keratinocytes and Fibroblasts. Evidence-Based Complementary and Alternative Medicine،Vol. 2013, no. 2013, pp.1-12.
https://search.emarefa.net/detail/BIM-468402
Modern Language Association (MLA)
Ramos-Jerz, Maria del R.…[et al.]. Persea americana Mill. Seed : Fractionation, Characterization, and Effects on Human Keratinocytes and Fibroblasts. Evidence-Based Complementary and Alternative Medicine No. 2013 (2013), pp.1-12.
https://search.emarefa.net/detail/BIM-468402
American Medical Association (AMA)
Ramos-Jerz, Maria del R.& Villanueva, Socorro& Jerz, Gerold& Winterhalter, Peter& Deters, Alexandra M.. Persea americana Mill. Seed : Fractionation, Characterization, and Effects on Human Keratinocytes and Fibroblasts. Evidence-Based Complementary and Alternative Medicine. 2013. Vol. 2013, no. 2013, pp.1-12.
https://search.emarefa.net/detail/BIM-468402
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-468402