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Modelling the Effects of Ageing Time of Starch on the Enzymatic Activity of Three Amylolytic Enzymes
Joint Authors
Guerra, Nelson Pérez
Pastrana Castro, Lorenzo
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-04-30
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Medicine
Information Technology and Computer Science
Abstract EN
The effect of increasing ageing time (t) of starch on the activity of three amylolytic enzymes (Termamyl, San Super, and BAN) was investigated.
Although all the enzymatic reactions follow michaelian kinetics, vmax decreased significantly (P<0.05) and KM increased (although not always significantly) with the increase in t.
The conformational changes produced in the starch chains as a consequence of the ageing seemed to affect negatively the diffusivity of the starch to the active site of the enzymes and the release of the reaction products to the medium.
A similar effect was observed when the enzymatic reactions were carried out with unaged starches supplemented with different concentrations of gelatine [G].
The inhibition in the amylolytic activities was best mathematically described by using three modified forms of the Michaelis-Menten model, which included a term to consider, respectively, the linear, exponential, and hyperbolic inhibitory effects of t and [G].
American Psychological Association (APA)
Guerra, Nelson Pérez& Pastrana Castro, Lorenzo. 2012. Modelling the Effects of Ageing Time of Starch on the Enzymatic Activity of Three Amylolytic Enzymes. The Scientific World Journal،Vol. 2012, no. 2012, pp.1-13.
https://search.emarefa.net/detail/BIM-469199
Modern Language Association (MLA)
Guerra, Nelson Pérez& Pastrana Castro, Lorenzo. Modelling the Effects of Ageing Time of Starch on the Enzymatic Activity of Three Amylolytic Enzymes. The Scientific World Journal No. 2012 (2012), pp.1-13.
https://search.emarefa.net/detail/BIM-469199
American Medical Association (AMA)
Guerra, Nelson Pérez& Pastrana Castro, Lorenzo. Modelling the Effects of Ageing Time of Starch on the Enzymatic Activity of Three Amylolytic Enzymes. The Scientific World Journal. 2012. Vol. 2012, no. 2012, pp.1-13.
https://search.emarefa.net/detail/BIM-469199
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-469199