Combined Beta-Agonists and Corticosteroids Do Not Inhibit Extracellular Matrix Protein Production In Vitro
Joint Authors
Burgess, Janette K.
Moir, Lyn M.
Black, Judith L.
Ge, Qi
Poniris, Maree H.
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-02-08
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Background.
Persistent asthma is characterized by airway remodeling.
Whereas we have previously shown that neither β2-agonists nor corticosteroids inhibit extracellular matrix (ECM) protein release from airway smooth muscle (ASM) cells, the effect of their combination is unknown and this forms the rationale for the present study.
Methods.
ASM cells from people with and without asthma were stimulated with TGFβ1 (1 ng/ml) with or without budesonide (10-8 M) and formoterol (10-10 and 10-8 M), and fibronectin expression and IL-6 release were measured by ELISA.
Bronchial rings from nonasthmatic individuals were incubated with TGFβ1 (1 ng/ml) with or without the drugs, and fibronectin expression was measured using immunohistochemistry.
Results.
Budesonide stimulated fibronectin deposition, in the presence or absence of TGFβ1, and this was partially reversed by formoterol (10-8 M) in both asthmatic and nonasthmatic cells.
Budesonide and formoterol in combination failed to inhibit TGFβ-induced fibronectin in either cell type.
A similar pattern of expression of fibronectin was seen in bronchial rings.
TGFβ1-induced IL-6 release was inhibited by the combination of drugs.
Conclusion.
Current combination asthma therapies are unable to prevent or reverse remodeling events regulated by ASM cells.
American Psychological Association (APA)
Ge, Qi& Poniris, Maree H.& Moir, Lyn M.& Black, Judith L.& Burgess, Janette K.. 2012. Combined Beta-Agonists and Corticosteroids Do Not Inhibit Extracellular Matrix Protein Production In Vitro. Journal of Allergy،Vol. 2012, no. 2012, pp.1-7.
https://search.emarefa.net/detail/BIM-469258
Modern Language Association (MLA)
Ge, Qi…[et al.]. Combined Beta-Agonists and Corticosteroids Do Not Inhibit Extracellular Matrix Protein Production In Vitro. Journal of Allergy No. 2012 (2012), pp.1-7.
https://search.emarefa.net/detail/BIM-469258
American Medical Association (AMA)
Ge, Qi& Poniris, Maree H.& Moir, Lyn M.& Black, Judith L.& Burgess, Janette K.. Combined Beta-Agonists and Corticosteroids Do Not Inhibit Extracellular Matrix Protein Production In Vitro. Journal of Allergy. 2012. Vol. 2012, no. 2012, pp.1-7.
https://search.emarefa.net/detail/BIM-469258
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-469258