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Protein Glycation in Diabetes as Determined by Mass Spectrometry
Joint Authors
Annunziata, Lapolla
Molin, Laura
Traldi, Pietro
Source
International Journal of Endocrinology
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-03-13
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Diabetes is a common endocrine disorder characterized by hyperglycemia leading to nonenzymatic glycation of proteins, responsible for chronic complications.
The development of mass spectrometric techniques able to give highly specific and reliable results in proteome field is of wide interest for physicians, giving them new tools to monitor the disease progression and the possible complications related to diabetes, as well as the effectiveness of therapeutic treatments.
This paper reports and discusses some of the data pertaining protein glycation in diabetic subjects obtained by matrix-assisted laser desorption ionization (MALDI) mass spectrometry (MS).
The preliminary studies carried out by in vitro protein glycation experiments show clear differences in molecular weight of glycated and unglycated proteins.
Then, the attention was focused on plasma proteins human serum albumin (HSA) and immunoglobulin G (IgG).
Enzymatic degradation products of in vitro glycated HSA were studied in order to simulate the in vivo enzymatic digestion of glycated species by the immunological system leading to the highly reactive advanced glycation end-products (AGEs) peptides.
Further studies led to the evaluation of glycated Apo A-I and glycated haemoglobin levels.
A different MALDI approach was employed for the identification of markers of disease in urine samples of healthy, diabetic, nephropathic, and diabetic-nephropathic subjects.
American Psychological Association (APA)
Annunziata, Lapolla& Molin, Laura& Traldi, Pietro. 2013. Protein Glycation in Diabetes as Determined by Mass Spectrometry. International Journal of Endocrinology،Vol. 2013, no. 2013, pp.1-11.
https://search.emarefa.net/detail/BIM-470030
Modern Language Association (MLA)
Annunziata, Lapolla…[et al.]. Protein Glycation in Diabetes as Determined by Mass Spectrometry. International Journal of Endocrinology No. 2013 (2013), pp.1-11.
https://search.emarefa.net/detail/BIM-470030
American Medical Association (AMA)
Annunziata, Lapolla& Molin, Laura& Traldi, Pietro. Protein Glycation in Diabetes as Determined by Mass Spectrometry. International Journal of Endocrinology. 2013. Vol. 2013, no. 2013, pp.1-11.
https://search.emarefa.net/detail/BIM-470030
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-470030