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Equine Arteritis Virus Does Not Induce Interferon Production in Equine Endothelial Cells : Identification of Nonstructural Protein 1 as a Main Interferon Antagonist
Joint Authors
Chen, Zhenhai
Yoo, Dongwan
Fang, Ying
Balasuriya, Udeni B. R.
Li, Yanhua
Go, Yun Young
Han, Mingyuan
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-05-25
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
The objective of this study was to investigate the effect of equine arteritis virus (EAV) on type I interferon (IFN) production.
Equine endothelial cells (EECs) were infected with the virulent Bucyrus strain (VBS) of EAV and expression of IFN-β was measured at mRNA and protein levels by quantitative real-time RT-PCR and IFN bioassay using vesicular stomatitis virus expressing the green fluorescence protein (VSV-GFP), respectively.
Quantitative RT-PCR results showed that IFN-β mRNA levels in EECs infected with EAV VBS were not increased compared to those in mock-infected cells.
Consistent with quantitative RT-PCR, Sendai virus- (SeV-) induced type I IFN production was inhibited by EAV infection.
Using an IFN-β promoter-luciferase reporter assay, we subsequently demonstrated that EAV nsps 1, 2, and 11 had the capability to inhibit type I IFN activation.
Of these three nsps, nsp1 exhibited the strongest inhibitory effect.
Taken together, these data demonstrate that EAV has the ability to suppress the type I IFN production in EECs and nsp1 may play a critical role to subvert the equine innate immune response.
American Psychological Association (APA)
Go, Yun Young& Li, Yanhua& Chen, Zhenhai& Han, Mingyuan& Yoo, Dongwan& Fang, Ying…[et al.]. 2014. Equine Arteritis Virus Does Not Induce Interferon Production in Equine Endothelial Cells : Identification of Nonstructural Protein 1 as a Main Interferon Antagonist. BioMed Research International،Vol. 2014, no. 2014, pp.1-13.
https://search.emarefa.net/detail/BIM-470843
Modern Language Association (MLA)
Go, Yun Young…[et al.]. Equine Arteritis Virus Does Not Induce Interferon Production in Equine Endothelial Cells : Identification of Nonstructural Protein 1 as a Main Interferon Antagonist. BioMed Research International No. 2014 (2014), pp.1-13.
https://search.emarefa.net/detail/BIM-470843
American Medical Association (AMA)
Go, Yun Young& Li, Yanhua& Chen, Zhenhai& Han, Mingyuan& Yoo, Dongwan& Fang, Ying…[et al.]. Equine Arteritis Virus Does Not Induce Interferon Production in Equine Endothelial Cells : Identification of Nonstructural Protein 1 as a Main Interferon Antagonist. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-13.
https://search.emarefa.net/detail/BIM-470843
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-470843