Recombinant Expression and Characterization of Human and Murine ACE2 : Species-Specific Activation of the Alternative Renin-Angiotensin-System
Joint Authors
Domenig, Oliver
Wagner, Bettina
Schwager, Cornelia
Peball, Bernhard
Schuster, Manfred
Janzek, Evelyne
Stranner, Stefan
Loibner, Hans
Poglitsch, Marko
Jungwirth, Helmut
Source
International Journal of Hypertension
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-02-28
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Angiotensin-converting enzyme 2 (ACE2) is a monocarboxypeptidase of the renin-angiotensin-system (RAS) which is known to cleave several substrates among vasoactive peptides.
Its preferred substrate is Angiotensin II, which is tightly involved in the regulation of important physiological functions including fluid homeostasis and blood pressure.
Ang 1–7, the main enzymatic product of ACE2, became increasingly important in the literature in recent years, as it was reported to counteract hypertensive and fibrotic actions of Angiotensin II via the MAS receptor.
The functional connection of ACE2, Ang 1–7, and the MAS receptor is also referred to as the alternative axis of the RAS.
In the present paper, we describe the recombinant expression and purification of human and murine ACE2 (rhACE2 and rmACE2).
Furthermore, we determined the conversion rates of rhACE2 and rmACE2 for different natural peptide substrates in plasma samples and discovered species-specific differences in substrate specificities, probably leading to functional differences in the alternative axis of the RAS.
In particular, conversion rates of Ang 1–10 to Ang 1–9 were found to be substantially different when applying rhACE2 or rmACE2 in vitro.
In contrast to rhACE2, rm ACE2 is substantially less potent in transformation of Ang 1–10 to Ang 1–9.
American Psychological Association (APA)
Poglitsch, Marko& Domenig, Oliver& Schwager, Cornelia& Stranner, Stefan& Peball, Bernhard& Janzek, Evelyne…[et al.]. 2012. Recombinant Expression and Characterization of Human and Murine ACE2 : Species-Specific Activation of the Alternative Renin-Angiotensin-System. International Journal of Hypertension،Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-471521
Modern Language Association (MLA)
Poglitsch, Marko…[et al.]. Recombinant Expression and Characterization of Human and Murine ACE2 : Species-Specific Activation of the Alternative Renin-Angiotensin-System. International Journal of Hypertension No. 2012 (2012), pp.1-8.
https://search.emarefa.net/detail/BIM-471521
American Medical Association (AMA)
Poglitsch, Marko& Domenig, Oliver& Schwager, Cornelia& Stranner, Stefan& Peball, Bernhard& Janzek, Evelyne…[et al.]. Recombinant Expression and Characterization of Human and Murine ACE2 : Species-Specific Activation of the Alternative Renin-Angiotensin-System. International Journal of Hypertension. 2012. Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-471521
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-471521