Characterization of Myelomonocytoid Progenitor Cells with Mesenchymal Differentiation Potential Obtained by Outgrowth from Pancreas Explants
Joint Authors
Roehrich, Marc-Estienne
Vassalli, Giuseppe
Source
Biotechnology Research International
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-08-21
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Information Technology and Computer Science
Abstract EN
Progenitor cells can be obtained by outgrowth from tissue explants during primary ex vivo tissue culture.
We have isolated and characterized cells outgrown from neonatal mouse pancreatic explants.
A relatively uniform population of cells showing a distinctive morphology emerged over time in culture.
This population expressed monocyte/macrophage and hematopoietic markers (CD11b+ and CD45+), and some stromal-related markers (CD44+ and CD29+), but not mesenchymal stem cell (MSC)-defining markers (CD90− and CD105−) nor endothelial (CD31−) or stem cell-associated markers (CD133− and stem cell antigen-1; Sca-1−).
Cells could be maintained in culture as a plastic-adherent monolayer in culture medium (MesenCult MSC) for more than 1 year.
Cells spontaneously formed sphere clusters “pancreatospheres” which, however, were nonclonal.
When cultured in appropriate media, cells differentiated into multiple mesenchymal lineages (fat, cartilage, and bone).
Positive dithizone staining suggested that a subset of cells differentiated into insulin-producing cells.
However, further studies are needed to characterize the endocrine potential of these cells.
These findings indicate that a myelomonocytoid population from pancreatic explant outgrowths has mesenchymal differentiation potential.
These results are in line with recent data onmonocyte-derivedmesenchymal progenitors (MOMPs).
American Psychological Association (APA)
Roehrich, Marc-Estienne& Vassalli, Giuseppe. 2012. Characterization of Myelomonocytoid Progenitor Cells with Mesenchymal Differentiation Potential Obtained by Outgrowth from Pancreas Explants. Biotechnology Research International،Vol. 2012, no. 2012, pp.1-12.
https://search.emarefa.net/detail/BIM-471628
Modern Language Association (MLA)
Roehrich, Marc-Estienne& Vassalli, Giuseppe. Characterization of Myelomonocytoid Progenitor Cells with Mesenchymal Differentiation Potential Obtained by Outgrowth from Pancreas Explants. Biotechnology Research International No. 2012 (2012), pp.1-12.
https://search.emarefa.net/detail/BIM-471628
American Medical Association (AMA)
Roehrich, Marc-Estienne& Vassalli, Giuseppe. Characterization of Myelomonocytoid Progenitor Cells with Mesenchymal Differentiation Potential Obtained by Outgrowth from Pancreas Explants. Biotechnology Research International. 2012. Vol. 2012, no. 2012, pp.1-12.
https://search.emarefa.net/detail/BIM-471628
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-471628