Control of Differentiation of Human Mesenchymal Stem Cells by Altering the Geometry of Nanofibers

Joint Authors

Shimizu, Harue
Fujita, Satoshi
Suye, Shin-ichiro

Source

Journal of Nanotechnology

Issue

Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-9, 9 p.

Publisher

Hindawi Publishing Corporation

Publication Date

2012-07-18

Country of Publication

Egypt

No. of Pages

9

Main Subjects

Engineering Sciences and Information Technology
Chemistry

Abstract EN

Effective differentiation of mesenchymal stem cells (MSCs) is required for clinical applications.

To control MSC differentiation, induction media containing different types of soluble factors have been used to date; however, it remains challenging to obtain a uniformly differentiated population of an appropriate quality for clinical application by this approach.

We attempted to develop nanofiber scaffolds for effective MSC differentiation by mimicking anisotropy of the extracellular matrix structure, to assess whether differentiation of these cells can be controlled by using geometrically different scaffolds.

We evaluated MSC differentiation on aligned and random nanofibers, fabricated by electrospinning.

We found that induction of MSCs into adipocytes was markedly more inhibited on random nanofibers than on aligned nanofibers.

In addition, adipoinduction on aligned nanofibers was also inhibited in the presence of mixed adipoinduction and osteoinduction medium, although osteoinduction was not affected by a change in scaffold geometry.

Thus, we have achieved localized control over the direction of differentiation through changes in the alignment of the scaffold even in the presence of a mixed medium.

These findings indicate that precise control of MSC differentiation can be attained by using scaffolds with different geometry, rather than by the conventional use of soluble factors in the medium.

American Psychological Association (APA)

Fujita, Satoshi& Shimizu, Harue& Suye, Shin-ichiro. 2012. Control of Differentiation of Human Mesenchymal Stem Cells by Altering the Geometry of Nanofibers. Journal of Nanotechnology،Vol. 2012, no. 2012, pp.1-9.
https://search.emarefa.net/detail/BIM-471629

Modern Language Association (MLA)

Fujita, Satoshi…[et al.]. Control of Differentiation of Human Mesenchymal Stem Cells by Altering the Geometry of Nanofibers. Journal of Nanotechnology No. 2012 (2012), pp.1-9.
https://search.emarefa.net/detail/BIM-471629

American Medical Association (AMA)

Fujita, Satoshi& Shimizu, Harue& Suye, Shin-ichiro. Control of Differentiation of Human Mesenchymal Stem Cells by Altering the Geometry of Nanofibers. Journal of Nanotechnology. 2012. Vol. 2012, no. 2012, pp.1-9.
https://search.emarefa.net/detail/BIM-471629

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references

Record ID

BIM-471629