Pronerve Growth Factor Induces Angiogenesis via Activation of TrkA : Possible Role in Proliferative Diabetic Retinopathy
Joint Authors
Nussbaum, Julian J.
Matragoon, Suraporn
Kumar, Parag
Elshaer, Sally L.
Abdelsaid, Mohammed A.
al-Azayzih, Ahmad
El-Remessy, Azza B.
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-08-12
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Proliferative diabetic retinopathy (PDR) is the leading cause of blindness in working age Americans.
We demonstrated that diabetes disturbs the homeostasis of nerve growth factor (NGF) resulting in accumulation of its precursor proNGF.
Increases in proNGF were positively correlated with progression of diabetic retinopathy, having the highest level in ocular fluids from PDR patients compared to nondiabetic patients.
Here, we attempted to evaluate the contribution and the possible mechanism of proNGF to PDR.
The angiogenic response of aqueous humor samples from PDR patients was examined in human retinal endothelial cells in the presence or absence of anti-proNGF antibody.
Additional cultures were treated with mutant-proNGF in the presence of specific pharmacological inhibitors of TrkA and p75NTR receptors.
PDR-aqueous humor samples exerted significant angiogenic response including cell proliferation, migration, and alignment into tube-like structures.
These effects were significantly reduced by anti-proNGF antibody but not by IgG.
Treatment of retinal endothelial cells with mutant-proNGF activated phosphorylation of TrkA and p38MAPK; however, it did not alter p75NTR expression.
Inhibition of TrkA but not p75NTR significantly reduced mutant-proNGF-induced cell proliferation, cell migration, and tube formation.
Taken together, these results provide evidence that proNGF can contribute to PDR at least in part via activation of TrkA.
American Psychological Association (APA)
Elshaer, Sally L.& Abdelsaid, Mohammed A.& al-Azayzih, Ahmad& Kumar, Parag& Matragoon, Suraporn& Nussbaum, Julian J.…[et al.]. 2013. Pronerve Growth Factor Induces Angiogenesis via Activation of TrkA : Possible Role in Proliferative Diabetic Retinopathy. Journal of Diabetes Research،Vol. 2013, no. 2013, pp.1-10.
https://search.emarefa.net/detail/BIM-471873
Modern Language Association (MLA)
Elshaer, Sally L.…[et al.]. Pronerve Growth Factor Induces Angiogenesis via Activation of TrkA : Possible Role in Proliferative Diabetic Retinopathy. Journal of Diabetes Research No. 2013 (2013), pp.1-10.
https://search.emarefa.net/detail/BIM-471873
American Medical Association (AMA)
Elshaer, Sally L.& Abdelsaid, Mohammed A.& al-Azayzih, Ahmad& Kumar, Parag& Matragoon, Suraporn& Nussbaum, Julian J.…[et al.]. Pronerve Growth Factor Induces Angiogenesis via Activation of TrkA : Possible Role in Proliferative Diabetic Retinopathy. Journal of Diabetes Research. 2013. Vol. 2013, no. 2013, pp.1-10.
https://search.emarefa.net/detail/BIM-471873
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-471873