Nanofibrous Chitosan-Polyethylene Oxide Engineered Scaffolds : A Comparative Study between Simulated Structural Characteristics and Cells Viability
Joint Authors
Kazemi Pilehrood, Mohammad
Harlin, Ali
Mirian, Mina
Dilamian, Mandana
Maleknia, Laleh
Nousiainen, Pertti
Sadeghi-Aliabadi, Hojjat
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-06-04
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
3D nanofibrous chitosan-polyethylene oxide (PEO) scaffolds were fabricated by electrospinning at different processing parameters.
The structural characteristics, such as pore size, overall porosity, pore interconnectivity, and scaffold percolative efficiency (SPE), were simulated by a robust image analysis.
Mouse fibroblast cells (L929) were cultured in RPMI for 2 days in the presence of various samples of nanofibrous chitosan/PEO scaffolds.
Cell attachments and corresponding mean viability were enhanced from 50% to 110% compared to that belonging to a control even at packed morphologies of scaffolds constituted from pores with nanoscale diameter.
To elucidate the correlation between structural characteristics within the depth of the scaffolds’ profile and cell viability, a comparative analysis was proposed.
This analysis revealed that larger fiber diameters and pore sizes can enhance cell viability.
On the contrary, increasing the other structural elements such as overall porosity and interconnectivity due to a simultaneous reduction in fiber diameter and pore size through the electrospinning process can reduce the viability of cells.
In addition, it was found that manipulation of the processing parameters in electrospinning can compensate for the effects of packed morphologies of nanofibrous scaffolds and can thus potentially improve the infiltration and viability of cells.
American Psychological Association (APA)
Kazemi Pilehrood, Mohammad& Dilamian, Mandana& Mirian, Mina& Sadeghi-Aliabadi, Hojjat& Maleknia, Laleh& Nousiainen, Pertti…[et al.]. 2014. Nanofibrous Chitosan-Polyethylene Oxide Engineered Scaffolds : A Comparative Study between Simulated Structural Characteristics and Cells Viability. BioMed Research International،Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-472320
Modern Language Association (MLA)
Kazemi Pilehrood, Mohammad…[et al.]. Nanofibrous Chitosan-Polyethylene Oxide Engineered Scaffolds : A Comparative Study between Simulated Structural Characteristics and Cells Viability. BioMed Research International No. 2014 (2014), pp.1-9.
https://search.emarefa.net/detail/BIM-472320
American Medical Association (AMA)
Kazemi Pilehrood, Mohammad& Dilamian, Mandana& Mirian, Mina& Sadeghi-Aliabadi, Hojjat& Maleknia, Laleh& Nousiainen, Pertti…[et al.]. Nanofibrous Chitosan-Polyethylene Oxide Engineered Scaffolds : A Comparative Study between Simulated Structural Characteristics and Cells Viability. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-472320
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-472320