PolyAlign : A Versatile LC-MS Data Alignment Tool for Landmark-Selected and -Automated Use
Joint Authors
Salmi, Jussi
Moulder, Robert
Aittokallio, Tero
Lahesmaa, Riitta
Vähämaa, Heidi
Hosia, Waltteri
Koskinen, Ville R.
Nevalainen, Olli S.
Source
International Journal of Proteomics
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-04-19
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Biology
Abstract EN
We present a versatile user-friendly software tool, PolyAlign, for the alignment of multiple LC-MS signal maps with the option of manual landmark setting or automated alignment.
One of the spectral images is selected as a reference map, and after manually setting the landmarks, the program warps the images using either polynomial or Hermite transformation.
The software provides an option for automated landmark finding.
The software includes a very fast zoom-in function synchronized between the images, which facilitate detecting correspondences between the adjacent images.
Such an interactive visual process enables the analyst to decide when the alignment is satisfactory and to correct known irregularities.
We demonstrate that the software provides significant improvements in the alignment of LC-MALDI data, with 10–15 landmark pairs, and it is also applicable to correcting electrospray LC-MS data.
The results with practical data show substantial improvement in peak alignment compared to MZmine, which was among the best analysis packages in a recent assessment.
The PolyAlign software is freely available and easily accessible as an integrated component of the popular MZmine software, and also as a simpler stand-alone Perl implementation to preview data and apply landmark directed polynomial transformation.
American Psychological Association (APA)
Vähämaa, Heidi& Koskinen, Ville R.& Hosia, Waltteri& Moulder, Robert& Nevalainen, Olli S.& Lahesmaa, Riitta…[et al.]. 2011. PolyAlign : A Versatile LC-MS Data Alignment Tool for Landmark-Selected and -Automated Use. International Journal of Proteomics،Vol. 2011, no. 2011, pp.1-9.
https://search.emarefa.net/detail/BIM-472521
Modern Language Association (MLA)
Vähämaa, Heidi…[et al.]. PolyAlign : A Versatile LC-MS Data Alignment Tool for Landmark-Selected and -Automated Use. International Journal of Proteomics No. 2011 (2011), pp.1-9.
https://search.emarefa.net/detail/BIM-472521
American Medical Association (AMA)
Vähämaa, Heidi& Koskinen, Ville R.& Hosia, Waltteri& Moulder, Robert& Nevalainen, Olli S.& Lahesmaa, Riitta…[et al.]. PolyAlign : A Versatile LC-MS Data Alignment Tool for Landmark-Selected and -Automated Use. International Journal of Proteomics. 2011. Vol. 2011, no. 2011, pp.1-9.
https://search.emarefa.net/detail/BIM-472521
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-472521