Rheumatoid Factor Positivity Is Associated with Increased Joint Destruction and Upregulation of Matrix Metalloproteinase 9 and Cathepsin K Gene Expression in the Peripheral Blood in Rheumatoid Arthritic Patients Treated with Methotrexate
Joint Authors
Demidova, Natalia V.
Karateev, Dmitry E.
Tchetina, Elena V.
Nasonov, Eugeny L.
Source
International Journal of Rheumatology
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-11-14
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
We evaluated changes in gene expression of mTOR, p21, caspase-3, ULK1, TNFα, matrix metalloproteinase (MMP)-9, and cathepsin K in the whole blood of rheumatoid arthritic (RA) patients treated with methotrexate (MTX) in relation to their rheumatoid factor status, clinical, immunological, and radiological parameters, and therapeutic response after a 24-month follow-up.
The study group consisted of 35 control subjects and 33 RA patients without previous history of MTX treatment.
Gene expression was measured using real-time RT-PCR.
Decreased disease activity in patients at the end of the study was associated with significant downregulation of TNFα expression.
Downregulation of mTOR was observed in seronegative patients, while no significant changes in the expression of p21, ULK1, or caspase-3 were noted in any RA patients at the end of the study.
The increase in erosion numbers observed in the seropositive patients at the end of the follow-up was accompanied by upregulation of MMP-9 and cathepsin K, while seronegative patients demonstrated an absence of significant changes in MMP-9 and cathepsin K expression and no increase in the erosion score.
Our results suggest that increased expression of MMP-9 and cathepsin K genes in the peripheral blood might indicate higher bone tissue destruction activity in RA patients treated with methotrexate.
The clinical study registration number is 0120.0810610.
American Psychological Association (APA)
Tchetina, Elena V.& Demidova, Natalia V.& Karateev, Dmitry E.& Nasonov, Eugeny L.. 2013. Rheumatoid Factor Positivity Is Associated with Increased Joint Destruction and Upregulation of Matrix Metalloproteinase 9 and Cathepsin K Gene Expression in the Peripheral Blood in Rheumatoid Arthritic Patients Treated with Methotrexate. International Journal of Rheumatology،Vol. 2013, no. 2013, pp.1-10.
https://search.emarefa.net/detail/BIM-473072
Modern Language Association (MLA)
Tchetina, Elena V.…[et al.]. Rheumatoid Factor Positivity Is Associated with Increased Joint Destruction and Upregulation of Matrix Metalloproteinase 9 and Cathepsin K Gene Expression in the Peripheral Blood in Rheumatoid Arthritic Patients Treated with Methotrexate. International Journal of Rheumatology No. 2013 (2013), pp.1-10.
https://search.emarefa.net/detail/BIM-473072
American Medical Association (AMA)
Tchetina, Elena V.& Demidova, Natalia V.& Karateev, Dmitry E.& Nasonov, Eugeny L.. Rheumatoid Factor Positivity Is Associated with Increased Joint Destruction and Upregulation of Matrix Metalloproteinase 9 and Cathepsin K Gene Expression in the Peripheral Blood in Rheumatoid Arthritic Patients Treated with Methotrexate. International Journal of Rheumatology. 2013. Vol. 2013, no. 2013, pp.1-10.
https://search.emarefa.net/detail/BIM-473072
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-473072