Therapeutic and Radiosensitizing Effects of Armillaridin on Human Esophageal Cancer Cells
Joint Authors
Chi, Chih-Wen
Chen, Chien-Chih
Chen, Yu-Jen
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-06-24
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Background.
Armillaridin (AM) is isolated from Armillaria mellea.
We examined the anticancer activity and radiosensitizing effect on human esophageal cancer cells.
Methods.
Human squamous cell carcinoma (CE81T/VGH and TE-2) and adenocarcinoma (BE-3 and SKGT-4) cell lines were cultured.
The MTT assay was used for cell viability.
The cell cycle was analyzed using propidium iodide staining.
Mitochondrial transmembrane potential was measured by DiOC6(3) staining.
The colony formation assay was performed for estimation of the radiation surviving fraction.
Human CE81T/VGH xenografts were established for evaluation of therapeutic activity in vivo.
Results.
AM inhibited the viability of four human esophageal cancer cell lines with an estimated concentration of 50% inhibition (IC50) which was 3.4–6.9 μM.
AM induced a hypoploid cell population and morphological alterations typical of apoptosis in cells.
This apoptosis induction was accompanied by a reduction of mitochondrial transmembrane potential.
AM accumulated cell cycle at G2/M phase and enhanced the radiosensitivity in CE81T/VGH cells.
In vivo, AM inhibited the growth of CE81T/VGH xenografts without significant impact on body weight and white blood cell counts.
Conclusion.
Armillaridin could inhibit growth and enhance radiosensitivity of human esophageal cancer cells.
There might be potential to integrate AM with radiotherapy for esophageal cancer treatment.
American Psychological Association (APA)
Chi, Chih-Wen& Chen, Chien-Chih& Chen, Yu-Jen. 2013. Therapeutic and Radiosensitizing Effects of Armillaridin on Human Esophageal Cancer Cells. Evidence-Based Complementary and Alternative Medicine،Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-473200
Modern Language Association (MLA)
Chi, Chih-Wen…[et al.]. Therapeutic and Radiosensitizing Effects of Armillaridin on Human Esophageal Cancer Cells. Evidence-Based Complementary and Alternative Medicine No. 2013 (2013), pp.1-8.
https://search.emarefa.net/detail/BIM-473200
American Medical Association (AMA)
Chi, Chih-Wen& Chen, Chien-Chih& Chen, Yu-Jen. Therapeutic and Radiosensitizing Effects of Armillaridin on Human Esophageal Cancer Cells. Evidence-Based Complementary and Alternative Medicine. 2013. Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-473200
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-473200