The Synthetic Compound Norcantharidin Induced Apoptosis in Mantle Cell Lymphoma In Vivo and In Vitro through the PI3K-Akt-NF-κB Signaling Pathway
Joint Authors
Li, Yan
Fang, Jie
Lv, Hongyan
Song, Xiaoning
Zhang, Jinqiao
Du, Hengfei
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-07-07
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
This study aimed to elucidate the antitumor activity of norcantharidin (NCTD) against human mantle cell lymphoma (MCL).
Cell proliferation and apoptosis were examined by MTS and flow cytometry.
Caspase-3, -8, and -9 activities were detected with a colorimetric caspase protease assay.
Apoptotic proteins—including PARP, cyclin D1, Bcl-2 family proteins, XIAP, and cIAP I—were studied by western blot.
The phosphoinositide 3 kinase (PI3K) inhibitor LY294002 was used to investigate the involvement of the PI3K/Akt signaling pathway.
In vivo studies were performed using Z138 cell xenografts in nude mice.
NCTD inhibited proliferation and induced apoptosis of Z138 and Mino cells, both in vitro and in vivo.
PI3Kp110α and p-Akt expressions were downregulated by NCTD treatment.
NCTD downregulated NF-κB activity by preventing NF-κB phosphorylation and nuclear translocation.
This effect was correlated with the suppression of NF-κB-regulated gene products, such as cyclin D1, BAX, survivin, Bcl-2, XIAP, and cIAP.
This phenomenon was blocked by the PI3K inhibitor LY294002.
Our results demonstrated that NCTD can induce growth arrest and apoptosis in MCL cells and that the mechanism may involve the PI3K/Akt/NF-κB signaling pathway.
NCTD may have therapeutic and/or adjuvant therapeutic applications in the treatment of MCL.
American Psychological Association (APA)
Lv, Hongyan& Li, Yan& Du, Hengfei& Fang, Jie& Song, Xiaoning& Zhang, Jinqiao. 2013. The Synthetic Compound Norcantharidin Induced Apoptosis in Mantle Cell Lymphoma In Vivo and In Vitro through the PI3K-Akt-NF-κB Signaling Pathway. Evidence-Based Complementary and Alternative Medicine،Vol. 2013, no. 2013, pp.1-11.
https://search.emarefa.net/detail/BIM-473399
Modern Language Association (MLA)
Lv, Hongyan…[et al.]. The Synthetic Compound Norcantharidin Induced Apoptosis in Mantle Cell Lymphoma In Vivo and In Vitro through the PI3K-Akt-NF-κB Signaling Pathway. Evidence-Based Complementary and Alternative Medicine No. 2013 (2013), pp.1-11.
https://search.emarefa.net/detail/BIM-473399
American Medical Association (AMA)
Lv, Hongyan& Li, Yan& Du, Hengfei& Fang, Jie& Song, Xiaoning& Zhang, Jinqiao. The Synthetic Compound Norcantharidin Induced Apoptosis in Mantle Cell Lymphoma In Vivo and In Vitro through the PI3K-Akt-NF-κB Signaling Pathway. Evidence-Based Complementary and Alternative Medicine. 2013. Vol. 2013, no. 2013, pp.1-11.
https://search.emarefa.net/detail/BIM-473399
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-473399