Hypoxia-Pretreated Human MSCs Attenuate Acute Kidney Injury through Enhanced Angiogenic and Antioxidative Capacities
Joint Authors
Huo, Yanhong
Liu, Lirui
Zhang, Wenbo
Wang, Yaping
Yang, Yonghong
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-07-15
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Hypoxia preconditioning has been confirmed as an effective strategy to enhance the therapeutic potentials of mesenchymal stem cells (MSCs), such as for myocardial ischemia.
However, whether hypoxia preconditioning would produce beneficial effects on MSC-based renal repair has not been demonstrated.
In the study, we aimed to determine the feasibility and efficacy of hypoxia preconditioning to enhance MSC-based therapy of acute kidney injury (AKI).
MSCs were isolated from human adipose tissues.
The paracrine effects of MSCs under normoxia and hypoxia were determined in vitro.
Rats of AKI were induced by kidney I/R surgery and randomly divided into three groups: I/R control receiving PBS injection; MSC group receiving normal MSC injection; hypoMSC group receiving hypoxia-preconditioned MSC injection.
It was demonstrated in vitro that paracrine effects of MSCs were significantly enhanced, especially angiogenic factors.
Dihydroethidium (DHE) staining showed that antioxidative activities of MSCs were significantly enhanced by hypoxia stimulation.
Vascularization, apoptosis, and histological injury were all significantly improved in hypoMSC injected group compared with that in control and MSC injected groups.
Finally, the renal function was also significantly improved in hypoMSC injected group compared with that in the other two groups as assessed by the serum creatinine and BUN levels.
American Psychological Association (APA)
Zhang, Wenbo& Liu, Lirui& Huo, Yanhong& Yang, Yonghong& Wang, Yaping. 2014. Hypoxia-Pretreated Human MSCs Attenuate Acute Kidney Injury through Enhanced Angiogenic and Antioxidative Capacities. BioMed Research International،Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-473476
Modern Language Association (MLA)
Zhang, Wenbo…[et al.]. Hypoxia-Pretreated Human MSCs Attenuate Acute Kidney Injury through Enhanced Angiogenic and Antioxidative Capacities. BioMed Research International No. 2014 (2014), pp.1-10.
https://search.emarefa.net/detail/BIM-473476
American Medical Association (AMA)
Zhang, Wenbo& Liu, Lirui& Huo, Yanhong& Yang, Yonghong& Wang, Yaping. Hypoxia-Pretreated Human MSCs Attenuate Acute Kidney Injury through Enhanced Angiogenic and Antioxidative Capacities. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-473476
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-473476