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Both ERKMAPK and TGF-BetaSmad Signaling Pathways Play a Role in the Kidney Fibrosis of Diabetic Mice Accelerated by Blood Glucose Fluctuation
Joint Authors
Peng, Xu
Cheng, Xiaoyun
Li, Yujuan
Liu, Xia
Gao, Wenke
Dang, Yongyan
Ye, Xiyun
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-07-14
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Background.
The notion that diabetic nephropathy is the leading cause of renal fibrosis prompted us to investigate the effects of blood glucose fluctuation (BGF) under high glucose condition on kidney in the mice.
Methods.
The diabetic and BGF animal models were established in this study.
Immunohistochemistry, Western blot, and RT-PCR analysis were applied to detect the expression of type I collagen, matrix metalloproteinase-1 (MMP1), metalloproteinase inhibitor 1 (TIMP1), transforming growth factor beta 1 (TGF-β1), phosphorylated-ERK, p38, smad2/3, and Akt.
Results.
BGF treatment increased type I collagen synthesis by two times compared with the control.
The expression of MMP1 was reduced markedly while TIMP1 synthesis was enhanced after BGF treatment.
ERK phosphorylation exhibits a significant increase in the mice treated with BGF.
Furthermore, BGF can markedly upregulate TGF-β1 expression.
The p-smad2 showed 2-fold increases compared with the only diabetic mice.
However, p-AKT levels were unchanged after BGF treatment.
Conclusions.
These data demonstrate that BGF can accelerate the trend of kidney fibrosis in diabetic mice by increasing collagen production and inhibiting collagen degradation.
Both ERK/MAPK and TGF-β/smad signaling pathways seem to play a role in the development of kidney fibrosis accelerated by blood glucose fluctuation.
American Psychological Association (APA)
Cheng, Xiaoyun& Gao, Wenke& Dang, Yongyan& Liu, Xia& Li, Yujuan& Peng, Xu…[et al.]. 2013. Both ERKMAPK and TGF-BetaSmad Signaling Pathways Play a Role in the Kidney Fibrosis of Diabetic Mice Accelerated by Blood Glucose Fluctuation. Journal of Diabetes Research،Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-473599
Modern Language Association (MLA)
Cheng, Xiaoyun…[et al.]. Both ERKMAPK and TGF-BetaSmad Signaling Pathways Play a Role in the Kidney Fibrosis of Diabetic Mice Accelerated by Blood Glucose Fluctuation. Journal of Diabetes Research No. 2013 (2013), pp.1-8.
https://search.emarefa.net/detail/BIM-473599
American Medical Association (AMA)
Cheng, Xiaoyun& Gao, Wenke& Dang, Yongyan& Liu, Xia& Li, Yujuan& Peng, Xu…[et al.]. Both ERKMAPK and TGF-BetaSmad Signaling Pathways Play a Role in the Kidney Fibrosis of Diabetic Mice Accelerated by Blood Glucose Fluctuation. Journal of Diabetes Research. 2013. Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-473599
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-473599