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Decursin Isolated from Angelica gigas Nakai Rescues PC12 Cells from Amyloid β-Protein-Induced Neurotoxicity through Nrf2-Mediated Upregulation of Heme Oxygenase-1 : Potential Roles of MAPK
Joint Authors
Zou, Li-yi
Lee, Yong-woo
Kim, Yong-ho
Wu, Tie
Li, Li
Du, Ji-kun
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-14, 14 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-05-09
Country of Publication
Egypt
No. of Pages
14
Main Subjects
Abstract EN
Decursin (D), purified from Angelica gigas Nakai, has been proven to exert neuroprotective property.
Previous study revealed that D reduced Aβ25‒35-induced cytotoxicity in PC12 cells.
Our study explored the underlying mechanisms by which D mediates its therapeutic effects in vitro.
Pretreatment of cells with D diminished intracellular generation of ROS in response to Aβ25‒35.
Western blot revealed that D significantly increased the expression and activity of HO-1, which was correlated with its protection against Aβ25‒35-induced injury.
Addition of ZnPP, an HO-1 competitive inhibitor, significantly attenuated its protective effect in Aβ25‒35-treated cells, indicating the vital role of HO-1 resistance to oxidative injury.
Moreover, D induced Nrf2 nuclear translocation, the upstream of HO-1 expression.
While investigating the signaling pathways responsible for HO-1 induction, D activated ERK and dephosphorylated p38 in PC12 cells.
Addition of U0126, a selective inhibitor of ERK, blocked D-induced Nrf2 activation and HO-1 induction and meanwhile reversed the protection of D against Aβ25‒35-induced cell death.
These findings suggest D augments cellular antioxidant defense capacity through both intrinsic free radical scavenging activity and activation of MAPK signal pathways that leads to Nrf2 activation, and subsequently HO-1 induction, thereby protecting the PC12 cells from Aβ25‒35-induced oxidative cytotoxicity.
American Psychological Association (APA)
Li, Li& Du, Ji-kun& Zou, Li-yi& Wu, Tie& Lee, Yong-woo& Kim, Yong-ho. 2013. Decursin Isolated from Angelica gigas Nakai Rescues PC12 Cells from Amyloid β-Protein-Induced Neurotoxicity through Nrf2-Mediated Upregulation of Heme Oxygenase-1 : Potential Roles of MAPK. Evidence-Based Complementary and Alternative Medicine،Vol. 2013, no. 2013, pp.1-14.
https://search.emarefa.net/detail/BIM-473803
Modern Language Association (MLA)
Li, Li…[et al.]. Decursin Isolated from Angelica gigas Nakai Rescues PC12 Cells from Amyloid β-Protein-Induced Neurotoxicity through Nrf2-Mediated Upregulation of Heme Oxygenase-1 : Potential Roles of MAPK. Evidence-Based Complementary and Alternative Medicine No. 2013 (2013), pp.1-14.
https://search.emarefa.net/detail/BIM-473803
American Medical Association (AMA)
Li, Li& Du, Ji-kun& Zou, Li-yi& Wu, Tie& Lee, Yong-woo& Kim, Yong-ho. Decursin Isolated from Angelica gigas Nakai Rescues PC12 Cells from Amyloid β-Protein-Induced Neurotoxicity through Nrf2-Mediated Upregulation of Heme Oxygenase-1 : Potential Roles of MAPK. Evidence-Based Complementary and Alternative Medicine. 2013. Vol. 2013, no. 2013, pp.1-14.
https://search.emarefa.net/detail/BIM-473803
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-473803