Liquid ChromatographyQuadrupole Time-of-Flight Mass Spectrometry for Identification of In Vitro and In Vivo Metabolites of Bornyl Gallate in Rats
Joint Authors
Meng, Xue
Zheng, Xiaohui
Jia, Pu
Wang, Shixiang
Bian, Liujiao
Liao, Sha
Yu, Jie
Wu, Yizhen
Zhao, Xinfeng
Lan, Wei
Source
Journal of Analytical Methods in Chemistry
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-03-27
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Bornyl gallate (BG) is a potential drug candidate synthesized by the reaction of two natural products, gallic acid and borneol.
Previous studies have strongly suggested that BG is worthy of further investigation due to antioxidant, antiatherosclerosis activities, and obvious activity of stimulating intersegmental vessel growth in zebrafish.
This work was designed to elucidate the metabolic profile of BG through analyzing its metabolites in vitro and in vivo by a chromatographic separation coupled with a mass spectrometry.
The metabolites of BG were characterized from the rat liver microsome incubation solution, as well as rat urine and plasma after oral administration.
Chromatographic separation was performed on an Agilent TC-C18 column (250 mm × 4.6 mm, 5 μm) with gradient elution using methanol and water containing 0.2% (V : V) formic acid as the mobile phase.
Metabolites identification involved analyzing the retention behaviors, changes of molecular weights and MS/MS fragment patterns of BG and the metabolites.
Five compounds were identified as isomers of hydroxylated BG metabolites in vitro.
The major metabolites of BG in rat urine and plasma proved to be BG-O-glucuronide and O-methyl BG-O-glucuronide.
The proposed method confirmed to be a reliable and sensitive alternative for characterizing metabolic pathways of BG.
American Psychological Association (APA)
Lan, Wei& Bian, Liujiao& Zhao, Xinfeng& Jia, Pu& Meng, Xue& Wu, Yizhen…[et al.]. 2013. Liquid ChromatographyQuadrupole Time-of-Flight Mass Spectrometry for Identification of In Vitro and In Vivo Metabolites of Bornyl Gallate in Rats. Journal of Analytical Methods in Chemistry،Vol. 2013, no. 2013, pp.1-10.
https://search.emarefa.net/detail/BIM-474355
Modern Language Association (MLA)
Lan, Wei…[et al.]. Liquid ChromatographyQuadrupole Time-of-Flight Mass Spectrometry for Identification of In Vitro and In Vivo Metabolites of Bornyl Gallate in Rats. Journal of Analytical Methods in Chemistry No. 2013 (2013), pp.1-10.
https://search.emarefa.net/detail/BIM-474355
American Medical Association (AMA)
Lan, Wei& Bian, Liujiao& Zhao, Xinfeng& Jia, Pu& Meng, Xue& Wu, Yizhen…[et al.]. Liquid ChromatographyQuadrupole Time-of-Flight Mass Spectrometry for Identification of In Vitro and In Vivo Metabolites of Bornyl Gallate in Rats. Journal of Analytical Methods in Chemistry. 2013. Vol. 2013, no. 2013, pp.1-10.
https://search.emarefa.net/detail/BIM-474355
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-474355