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Sublethal Oxidative Stress Induces the Premature Senescence of Human Mesenchymal Stem Cells Derived from Endometrium
Joint Authors
Borodkina, Aleksandra
Burova, Elena
Shatrova, Alla
Nikolsky, Nikolay
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-08-25
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Biology
Abstract EN
The specific responses of mesenchymal stem cells to oxidative stress may play a crucial role in regulation of tissue homeostasis as well as regeneration of organs after oxidative injury.
The responses of human endometrium-derived mesenchymal stem cells (hMESCs) to oxidative stress remain still unknown.
Herein, we examined the impact of H2O2 on cell viability, induction of premature senescence, and apoptosis.
hMESCs were highly resistant to H2O2 compared with human diploid fibroblasts.
To test a hypothesis whether hMESCs may undergo oxidative stress-induced premature senescence, cells were briefly exposed to the sublethal H2O2 doses.
H2O2-treated cells were permanently arrested, lost Ki67 proliferation marker, and exhibited a senescent phenotype including cell hypertrophy and increased SA-β-Gal activity.
Additionally, in stressed cells the expression levels of p21Cip1, SOD1, SOD2, and GPX1 were elevated.
hMESCs survived under stress were not able to resume proliferation, indicating the irreversible loss of proliferative potential.
While the low H2O2 doses promoted senescence in hMESCs, the higher H2O2 doses induced also apoptosis in a part of the cell population.
Of note, senescent hMESCs exhibited high resistance to apoptosis.
Thus, we have demonstrated for the first time that hMESCs may enter a state of premature senescence in response to sublethal oxidative stress.
American Psychological Association (APA)
Burova, Elena& Borodkina, Aleksandra& Shatrova, Alla& Nikolsky, Nikolay. 2013. Sublethal Oxidative Stress Induces the Premature Senescence of Human Mesenchymal Stem Cells Derived from Endometrium. Oxidative Medicine and Cellular Longevity،Vol. 2013, no. 2013, pp.1-12.
https://search.emarefa.net/detail/BIM-474474
Modern Language Association (MLA)
Burova, Elena…[et al.]. Sublethal Oxidative Stress Induces the Premature Senescence of Human Mesenchymal Stem Cells Derived from Endometrium. Oxidative Medicine and Cellular Longevity No. 2013 (2013), pp.1-12.
https://search.emarefa.net/detail/BIM-474474
American Medical Association (AMA)
Burova, Elena& Borodkina, Aleksandra& Shatrova, Alla& Nikolsky, Nikolay. Sublethal Oxidative Stress Induces the Premature Senescence of Human Mesenchymal Stem Cells Derived from Endometrium. Oxidative Medicine and Cellular Longevity. 2013. Vol. 2013, no. 2013, pp.1-12.
https://search.emarefa.net/detail/BIM-474474
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-474474