Berry and Citrus Phenolic Compounds Inhibit Dipeptidyl Peptidase IV : Implications in Diabetes Management
Joint Authors
Fan, Junfeng
Yousef, Gad
Johnson, Michelle H.
de Mejia, Elvira Gonzalez
Lila, Mary Ann
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-08-29
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
Beneficial health effects of fruits and vegetables in the diet have been attributed to their high flavonoid content.
Dipeptidyl peptidase IV (DPP-IV) is a serine aminopeptidase that is a novel target for type 2 diabetes therapy due to its incretin hormone regulatory effects.
In this study, well-characterized anthocyanins (ANC) isolated from berry wine blends and twenty-seven other phenolic compounds commonly present in citrus, berry, grape, and soybean, were individually investigated for their inhibitory effects on DPP-IV by using a luminescence assay and computational modeling.
ANC from blueberry-blackberry wine blends strongly inhibited DPP-IV activity (IC50, 0.07 ± 0.02 to >300 μM).
Of the twenty-seven phenolics tested, the most potent DPP-IV inhibitors were resveratrol (IC50, 0.6 ± 0.4 nM), luteolin (0.12 ± 0.01 μM), apigenin (0.14 ± 0.02 μM), and flavone (0.17 ± 0.01 μM), with IC50 values lower than diprotin A (4.21 ± 2.01 μM), a reference standard inhibitory compound.
Analyses of computational modeling showed that resveratrol and flavone were competitive inhibitors which could dock directly into all three active sites of DPP-IV, while luteolin and apigenin docked in a noncompetitive manner.
Hydrogen bonding was the main binding mode of all tested phenolic compounds with DPP-IV.
These results indicate that flavonoids, particularly luteolin, apigenin, and flavone, and the stilbenoid resveratrol can act as naturally occurring DPP-IV inhibitors.
American Psychological Association (APA)
Fan, Junfeng& Johnson, Michelle H.& Lila, Mary Ann& Yousef, Gad& de Mejia, Elvira Gonzalez. 2013. Berry and Citrus Phenolic Compounds Inhibit Dipeptidyl Peptidase IV : Implications in Diabetes Management. Evidence-Based Complementary and Alternative Medicine،Vol. 2013, no. 2013, pp.1-13.
https://search.emarefa.net/detail/BIM-474822
Modern Language Association (MLA)
Fan, Junfeng…[et al.]. Berry and Citrus Phenolic Compounds Inhibit Dipeptidyl Peptidase IV : Implications in Diabetes Management. Evidence-Based Complementary and Alternative Medicine No. 2013 (2013), pp.1-13.
https://search.emarefa.net/detail/BIM-474822
American Medical Association (AMA)
Fan, Junfeng& Johnson, Michelle H.& Lila, Mary Ann& Yousef, Gad& de Mejia, Elvira Gonzalez. Berry and Citrus Phenolic Compounds Inhibit Dipeptidyl Peptidase IV : Implications in Diabetes Management. Evidence-Based Complementary and Alternative Medicine. 2013. Vol. 2013, no. 2013, pp.1-13.
https://search.emarefa.net/detail/BIM-474822
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-474822